| 筛选转基因动物的新方法——PCR及其产物测序法 |
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| 引用本文： | 何泉,李鹏,姜立群,陈兰英.筛选转基因动物的新方法——PCR及其产物测序法[J].生物化学与生物物理进展,1997,24(6):552-554. |
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| 作者姓名： | 何泉 李鹏 姜立群 陈兰英 |
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| 作者单位： | 中国医学科学院阜外心血管病医院中国协和医科大学心血管病研究所, 北京100037;中国医学科学院阜外心血管病医院中国协和医科大学心血管病研究所, 北京100037;中国医学科学院阜外心血管病医院中国协和医科大学心血管病研究所, 北京100037;中国医学科学院阜外心血管病医院中国协和医科大学心血管病研究所, 北京100037 |
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| 基金项目： | 国家自然科学基金(39570297)和中国医学科学院基金资助课题. |
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| 摘 要： | 用特异性引物对肌球蛋白轻链2启动子(myosin light chain-2,MLC2)-糜酶融合基因的转基因新生鼠鼠尾DNA进行PCR筛选, 低熔点琼脂糖凝胶电泳回收阳性样品PCR所扩出的DNA条带,纯化后用同一对引物中的一个进行单引物PCR测序,与所转外源基因序列比较,进一步确定整合有外源基因的阳性鼠.PCR及PCR 产物测序法检测转基因动物具有操作方便,灵敏度高及特异性强等优点.
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| 关 键 词： | PCR，转基因动物筛选，PCR测序 |
| 收稿时间： | 1996/10/15 0:00:00 |
| 修稿时间： | 1/6/1997 12:00:00 AM |
A New Method of Screening Transgenic Animal: PCR and Its Products Sequencing |
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| HE Quan,LI Peng,JIANG Liqun and CHEN Lanying.A New Method of Screening Transgenic Animal: PCR and Its Products Sequencing[J].Progress In Biochemistry and Biophysics,1997,24(6):552-554. |
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| Authors: | HE Quan LI Peng JIANG Liqun and CHEN Lanying |
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| Institution: | Fu Wai Hospital, Chinese Academy of Medical Sciences, Cardiovascular Institute, Peking Union Medical University, Beijing 100037, China;Fu Wai Hospital, Chinese Academy of Medical Sciences, Cardiovascular Institute, Peking Union Medical University, Beijing 100037, China;Fu Wai Hospital, Chinese Academy of Medical Sciences, Cardiovascular Institute, Peking Union Medical University, Beijing 100037, China;Fu Wai Hospital, Chinese Academy of Medical Sciences, Cardiovascular Institute, Peking Union Medical University, Beijing 100037, China |
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| Abstract: | The primary detection of the MLC2-chymase fusion gene transgenic mice was carried out by means of polymerase chain reaction(PCR) with two specific primers, which span across MLC2 promoter region and chymase structure gene region. The DNA electrophoretic bands of PCR products were recovered and purified, then sequenced by PCR sequencing method with one of the two primers. Final determination of the transgenic mice was conducted through comparing the sequencing results with sequences of transfered gene. This method is convenient, effective and specific. |
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| Keywords: | PCR screening transgenic animal PCR sequencing |
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