| DADS对DJ-1核定位高表达人白血病HL-60细胞的影响 |
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| 引用本文： | 岳海燕,秦晶,王文松,杨叶宁,易岚,王娟,唐玉娴,何洁,苏琦,谭晖.DADS对DJ-1核定位高表达人白血病HL-60细胞的影响[J].生物化学与生物物理进展,2019,46(2):201-210. |
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| 作者姓名： | 岳海燕 秦晶 王文松 杨叶宁 易岚 王娟 唐玉娴 何洁 苏琦 谭晖 |
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| 作者单位： | 南华大学肿瘤所，衡阳 421001,常德市第一人民医院病理科，常德 415000,南华大学肿瘤所，衡阳 421001,南华大学肿瘤所，衡阳 421001,南华大学肿瘤所，衡阳 421001,南华大学肿瘤所，衡阳 421001,南华大学肿瘤所，衡阳 421001,南华大学肿瘤所，衡阳 421001,南华大学肿瘤所，衡阳 421001,南华大学肿瘤所，衡阳 421001 |
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| 基金项目： | 湖南省重点学科建设及科技创新经费 [(2011)76]， 国家自然科学基金 (81100375,81400117)和湖南省高校创新平台基金(12K094)资助项目. |
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| 摘 要： | 最近研究表明，DJ-1在许多肿瘤中过表达，而且DJ-1的核表达与肿瘤的生物学行为有关. 本文主要研究二烯丙基二硫（DADS）对DJ-1核定位高表达人白血病HL-60细胞生物行为学的影响，阐明DJ-1在细胞核内的功能，为临床诊断及治疗过程提供一个潜在的治疗靶点. 通过基因转染技术建立核内高表达HL-60细胞株（DJ-1/HL-60），利用软琼脂集落形成实验、 MTT法、间接免疫荧光细胞化学实验、硝基蓝四氮唑( NBT)还原比色实验评估HL-60细胞的增殖与分化，DJ-1核定位过表达促进HL-60细胞的增殖并抑制其分化. Transwell迁移侵袭小室实验表明DJ-1核定位过表达可以促进HL-60的迁移和侵袭能力. Western blot结果表明DADS具有抑制HL-60细胞中核内DJ-1蛋白表达的能力. 说明DJ-1核定位高表达具有促进HL-60细胞增殖和迁移侵袭及抑制HL-60细胞分化的作用，DADS可以诱导DJ-1核定位高表达HL-60细胞分化及抑制迁移侵袭， DJ-1核定位高表达可减弱DADS抑制HL-60细胞增殖的作用.
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| 关 键 词： | HL-60细胞 二烯丙基二硫 DJ-1 细胞核定位 分化 |
| 收稿时间： | 2018/7/13 0:00:00 |
| 修稿时间： | 2018/12/19 0:00:00 |
DADS on The Biological Behavior of Human Leukemia HL-60 Cells With Nuclear Localization and Overexpression of DJ-1 |
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| YUE Hai-Yan,QIN Jing,WANG Wen-Song,YANG Ye-Ning,YI Lan,WANG Juan,TANG Yu-Xian,HE Jie,SU Qi and TAN Hui.DADS on The Biological Behavior of Human Leukemia HL-60 Cells With Nuclear Localization and Overexpression of DJ-1[J].Progress In Biochemistry and Biophysics,2019,46(2):201-210. |
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| Authors: | YUE Hai-Yan QIN Jing WANG Wen-Song YANG Ye-Ning YI Lan WANG Juan TANG Yu-Xian HE Jie SU Qi and TAN Hui |
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| Institution: | Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China , Hengyang 421001, China,Department of Pathology, The First People''s Hospital of Changde City, Changde 415000, China,Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China , Hengyang 421001, China,Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China , Hengyang 421001, China,Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China , Hengyang 421001, China,Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China , Hengyang 421001, China,Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China , Hengyang 421001, China,Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China , Hengyang 421001, China,Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China , Hengyang 421001, China,Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan Province, Cancer Research Institute, University of South China , Hengyang 421001, China |
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| Abstract: | Recent studies have revealed that DJ-1 is overexpressed in multiple forms of human tumors, and nuclear overexpression of DJ-1 is correlated with the biological behavior of tumor. The purpose of the present study was to test for possible DADS effects on Human Leukemia HL-60 cells which DJ-1 is overexpressed, and to clarify the function of DJ-1 in the nucleus. Gene transfection technology was used to establish stable HL-60 cell lines with nuclear overexpression of DJ-1 (DJ-1/HL-60). Soft agar colony formation, MTT, NBT and indirect immunofluorescence were used to evaluate the proliferation, differentiation, migration and invasion of HL-60 cells. The nuclear overexpression of DJ-1 protein may promote the proliferation and weaken the differentiation of HL-60 cells. Transwell migration and invasion assays showed that the nuclear localization and overexpression of DJ-1 can promote the migration and invasion capability of HL-60. Western blot analysis revealed that the expression of DJ-1 was inhibited in HL-60 cells with nuclear overexpression of DJ-1 after treatment with DADS. These results defined the impacts of DJ-1 on biological behavior of the Human Leukemia HL-60 cells by DADS, the proliferation, migration and invasion were inhibited and the differentiation was induced in HL-60 cells with nuclear overexpression of DJ-1, after treatment with DADS. |
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| Keywords: | HL-60 diallyl disulfide DJ-1 nuclear localization differentiation |
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