| 基因组消减杂交技术及其应用 |
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| 引用本文： | 侯萍,吴旻.基因组消减杂交技术及其应用[J].生物化学与生物物理进展,1995,22(5):394-397. |
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| 作者姓名： | 侯萍 吴旻 |
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| 作者单位： | 中国医学科学院肿瘤研究所;中国医学科学院肿瘤研究所 |
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| 摘 要： | 基因组消减杂交是通过突变型（驱赶DNA,driver)与野生型(检测DNA,tester)基因组DNA之间的差异比较来分离和鉴定突变基因的方法，该方法具有简便、快速、敏感、经济等特点，已被广泛应用于分离和鉴定肿瘤缺失和重排基因，制备多态位点探针，分离遗传性疾病、病毒感染性疾病的致病基因和基因诊断等领域．文章对这一新技术的建立和发展、实验策略、应用实例、目前存在的问题和发展前景等方面做了简要的综述．
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| 关 键 词： | 消减杂交 基因缺失 核酸酶SI PCR |
| 收稿时间： | 9/5/1994 12:00:00 AM |
| 修稿时间： | 1994/11/29 0:00:00 |
The Method and Applications of Genomic Subtraction |
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| Hou Ping and Wu Min.The Method and Applications of Genomic Subtraction[J].Progress In Biochemistry and Biophysics,1995,22(5):394-397. |
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| Authors: | Hou Ping and Wu Min |
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| Institution: | National Laboratory of Molecular Oncology and Department of Cell Biology, Cancer Institute, CAMS-PUMC;National Laboratory of Molecular Oncology and Department of Cell Biology, Cancer Institute, CAMS-PUMC |
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| Abstract: | Genomic subtraction is an efficient and economical method for isolating sequences pressent in one genomic DNA population(tester)but absent in another(driver).It proved widely applicable to detecting genomic abnormalities in cancer,probes for polymorphic loci and acquisition of new sequences in infectious diseases of viral organism. The fundation and development of genomic subtraction,experimental strategy,applications, problems in present and promise in future are described. |
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| Keywords: | subtractive hybridization deleted gene PCR nuclease S1 |
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