| 一个肝癌相关长链非编码RNA的克隆及序列分析 |
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| 引用本文： | 唐 珂,魏 芳,孛昊,黄宏斌,张文玲,龚朝建,李夏雨,宋亚莉,廖前进,彭淑平,向娟娟,周鸣,马健,李小玲,熊炜,李勇,曾朝阳,李桂源.一个肝癌相关长链非编码RNA的克隆及序列分析[J].生物化学与生物物理进展,2014,41(2):153-162. |
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| 作者姓名： | 唐 珂 魏 芳 孛昊 黄宏斌 张文玲 龚朝建 李夏雨 宋亚莉 廖前进 彭淑平 向娟娟 周鸣 马健 李小玲 熊炜 李勇 曾朝阳 李桂源 |
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| 作者单位： | 中南大学湘雅医学院附属肿瘤医院，湖南省肿瘤医院，长沙 410013；中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078；国防科技大学信息系统工程重点实验室，长沙 410073,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学湘雅三医院疾病基因组研究中心，湖南省非可控性炎症与肿瘤重点实验室，长沙 410013,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学湘雅医学院附属肿瘤医院，湖南省肿瘤医院，长沙 410013,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学湘雅医学院附属肿瘤医院，湖南省肿瘤医院，长沙 410013；中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078; 中南大学湘雅三医院疾病基因组研究中心，湖南省非可控性炎症与肿瘤重点实验室，长沙 410013,Department of Biochemistry and Molecular Biology, Center for Genetics and Molecular Medicine, School of Medicine,University of Louisville, Louisville, KY 40202, USA,中南大学湘雅医学院附属肿瘤医院，湖南省肿瘤医院，长沙 410013；中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078; 中南大学湘雅三医院疾病基因组研究中心，湖南省非可控性炎症与肿瘤重点实验室，长沙 410013,中南大学湘雅医学院附属肿瘤医院，湖南省肿瘤医院，长沙 410013；中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078; 中南大学湘雅三医院疾病基因组研究中心，湖南省非可控性炎症与肿瘤重点实验室，长沙 410013 |
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| 基金项目： | 国家自然科学基金(81372907, 81172189, 81171930, 81272298, 81272255和91229122), 湖南省自然科学基金(14JJ1010), 霍英东高校青年教师基金(121036), 中央高校基本科研业务费专项基金(2011JQ020)及中南大学博士后科学基金资助项目 |
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| 摘 要： | 最近我们利用新一代测序(next generation sequencing,NGS)技术对肝细胞癌(hepatocellular carcinoma,HCC)患者活检标本及正常对照肝组织样品进行高通量RNA测序(RNA-sequencing,RNA-Seq),在肝癌样品中染色体11q13.1区域检测到几个相邻的RNA-Seq信号峰,而在正常对照组织中没有检测到,且该染色体区域目前尚无已知基因登录,提示这几个RNA-Seq峰可能代表一个或多个未知的新基因.以此为线索,证实这几个RNA-Seq峰来自同一个新基因,并克隆了该基因全长序列,在克隆该基因全长序列时,发现该基因编码的RNA存在多种剪接形式,最长的转录本为3 562 bp.将该基因编码的12条代表性RNA转录本序列递交到美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)的GenBank数据库中,GenBank ID号分别为KC136297~KC136308.该基因编码的RNA没有发现明显的开放阅读框(open reading fragment,ORF),提示该基因可能编码长链非编码RNA(long non-coding RNA,lncRNA).为了探讨该lncRNA基因可能的转录调控机制,我们用生物信息学方法预测了该lncRNA基因潜在启动子区域,发现在其转录起始位点上游-719~-469 bp处有一个潜在的启动子,其中包含7个Sp1、1个STAT5和1个EGR1转录因子结合位点.该lncRNA在肝细胞癌发生发展过程中的作用机制值得进一步深入研究.
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| 关 键 词： | 长链非编码RNA 肝细胞癌 染色体q. 基因克隆 生物信息学 |
| 收稿时间： | 2012/12/18 0:00:00 |
| 修稿时间： | 4/9/2013 12:00:00 AM |
Cloning and Functional Characterization of a Novel Long Non-coding RNA Gene Associated With Hepatocellular Carcinoma |
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| TANG Ke,WEI Fang,BO Hao,HUANG Hong-Bin,ZHANG Wen-Ling,GONG Zhao-Jian,LI Xia-Yu,SONG Ya-Li,LIAO Qian Jin,PENG Shu-Ping,XIANG Juan-Juan,ZHOU Ming,MA Jian,LI Xiao-Ling,XIONG Wei,LI Yong,ZENG Zhao-Yang and LI Gui-Yuan.Cloning and Functional Characterization of a Novel Long Non-coding RNA Gene Associated With Hepatocellular Carcinoma[J].Progress In Biochemistry and Biophysics,2014,41(2):153-162. |
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| Authors: | TANG Ke WEI Fang BO Hao HUANG Hong-Bin ZHANG Wen-Ling GONG Zhao-Jian LI Xia-Yu SONG Ya-Li LIAO Qian Jin PENG Shu-Ping XIANG Juan-Juan ZHOU Ming MA Jian LI Xiao-Ling XIONG Wei LI Yong ZENG Zhao-Yang and LI Gui-Yuan |
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| Institution: | Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya School of Medicine,Central South University, Changsha 410011, China;Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China; Key Laboratory of Information System Engineering, National University of Defense Technology, Changsha 410073, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center,The Third Xiangya Hospital, Central South University, Changsha 410013, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya School of Medicine,Central South University, Changsha 410011, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China,Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya School of Medicine,Central South University, Changsha 410011, China;Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center,The Third Xiangya Hospital, Central South University, Changsha 410013, China,Department of Biochemistry and Molecular Biology, Center for Genetics and Molecular Medicine, School of Medicine,University of Louisville, Louisville, KY 40202, USA,Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya School of Medicine,Central South University, Changsha 410011, China;Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center,The Third Xiangya Hospital, Central South University, Changsha 410013, China and Hunan Provincial Tumor Hospital and the Affiliated Tumor Hospital of Xiangya School of Medicine,Central South University, Changsha 410011, China;Key Laboratory of Carcinogenesis of Ministry of Health and Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education,Cancer Research Institute, Central South University, Changsha 410078, China; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center,The Third Xiangya Hospital, Central South University, Changsha 410013, China |
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| Abstract: | |
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| Keywords: | long non-coding RNA hepatocellular carcinoma chromosome 11q13 1 gene cloning bioinformatics |
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