| 利用凝胶介质提高古菌DNA结合蛋白晶体的衍射分辨率 |
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| 引用本文： | 王刚刚,杨海涛,饶子和.利用凝胶介质提高古菌DNA结合蛋白晶体的衍射分辨率[J].生物化学与生物物理进展,2003,30(3):471-473. |
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| 作者姓名： | 王刚刚 杨海涛 饶子和 |
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| 作者单位： | 清华大学生物科学与技术系,教育部蛋白质科学重点实验室,北京,100084 |
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| 基金项目： | 国家重点基础研究发展规划项目“973”（G1999075602）. |
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| 摘 要： | 在凝胶介质中结晶了极端嗜酸热菌Sulfolobus shibatae DNA结合蛋白Ssh10b，与没有凝胶介质的条件下相比，晶体的出晶速度减缓，晶体的外形变得更规则，晶胞的C轴变短，晶体衍射分辨率从0.65 nm提高到了0.35 nm.
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| 关 键 词： | Sulfolobus shibatae DNA结合蛋白 凝胶介质 分辨率 |
| 收稿时间： | 2002/10/9 0:00:00 |
| 修稿时间： | 2002年10月9日 |
Improvement on Diffraction Resolution of an Archaeon DNA Binding Protein by Gel Medium |
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| WANG Gang-Gang,YANG Hai-Tao and RAO Zi-He.Improvement on Diffraction Resolution of an Archaeon DNA Binding Protein by Gel Medium[J].Progress In Biochemistry and Biophysics,2003,30(3):471-473. |
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| Authors: | WANG Gang-Gang YANG Hai-Tao and RAO Zi-He |
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| Institution: | Ministry of Education Laboratory of Protein Science & Laboratory of Structural Biology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China;Ministry of Education Laboratory of Protein Science & Laboratory of Structural Biology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China;Ministry of Education Laboratory of Protein Science & Laboratory of Structural Biology, Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China |
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| Abstract: | A small DNA-binding protein (Ssh10b) from the hyperthermophilic archaeon Sulfolobus shibatae was crystallized. The crystals could only diffract to 0.65 nm. And gel medium was used to improve the diffraction resolution. Gel adding led to slower speed of crystallization, change of crystal morphology and shortening of C axis of unit cell. The crystals grown in gel medium could diffract to 0.35 nm, and a set of data was collected. |
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| Keywords: | Sulfolobus shibatae DNA binding protein gel medium resolution |
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