| 超氧化物歧化酶切割超螺旋DNA的活性 |
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| 引用本文： | 凌俊,阮康成,刘望夷.超氧化物歧化酶切割超螺旋DNA的活性[J].生物化学与生物物理进展,1997,24(2):183-184. |
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| 作者姓名： | 凌俊 阮康成 刘望夷 |
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| 作者单位： | 中国科学院上海生物化学研究所，上海 200031;中国科学院上海生物化学研究所，上海 200031;中国科学院上海生物化学研究所，上海 200031 |
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| 基金项目： | 国家自然科学基金和中国科学院基金资助项目. |
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| 摘 要： | 在体外系统中，发现超氧化物歧化酶(SOD)具有切割超螺旋DNA的活性. 猪血和牛血Cu/Zn-SOD以及烟草Mn-SOD都能将超螺旋DNA转变为非超螺旋结构的缺刻环状DNA，进一步产生线状DNA. 它们只作用于超螺旋DNA而不作用于线状DNA. 这个事实排除了SOD样品中污染核酸酶的可能性. 用H2O2、胍基抑制或蛋白酶降解的实验结果表明，这两种酶的活性中心处于酶蛋白的不同部位.
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| 关 键 词： | 猪血Cu/Zn-SOD，牛血Cu/Zn-SOD，烟草Mn-SOD，超螺旋DNA切割活性 |
| 收稿时间： | 1996/11/25 0:00:00 |
| 修稿时间： | 1997/1/19 0:00:00 |
Activity of Superoxide Dismutase on Cleaving Supercoiled Double-Stranded DNA |
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| LING Jun,RUAN Kangcheng and LIU Wangyi.Activity of Superoxide Dismutase on Cleaving Supercoiled Double-Stranded DNA[J].Progress In Biochemistry and Biophysics,1997,24(2):183-184. |
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| Authors: | LING Jun RUAN Kangcheng and LIU Wangyi |
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| Institution: | Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China;Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China;Shanghai Institute of Biochemistry, Academia Sinica, Shanghai 200031, China |
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| Abstract: | Several superoxide dismutases (SODs), such as porcine and bovine erythrocyte Cu/Zn-SODs and N.tabacum Mn-SOD, were found to exhibit the activity to cleave supercoiled DNA in vitro. They converted supercoiled DNA into nicked and further into linear form. They did not act on linear double-stranded DNA. Activity assays after they were inactivated by H2O2 or guanidine and hydrolyzed by proteases suggested that these two activities of dismuting O·2 and cleaving supercoiled-DNA involved in different sites in SODs. |
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| Keywords: | porcine erythrocyte Cu/Zn-SOD bovine erythrocyte Cu/Zn-SOD N tabacum Mn-SOD supercoiled DNA-cleaving activity |
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