| OsCOI1,水稻中一个受茉莉酸甲酯和脱落酸诱导表达的F-box家族基因 |
| |
| 引用本文： | 胡廷章,王维平,曹凯鸣,王喜萍.OsCOI1,水稻中一个受茉莉酸甲酯和脱落酸诱导表达的F-box家族基因[J].生物化学与生物物理进展,2006,33(4). |
| |
| 作者姓名： | 胡廷章 王维平 曹凯鸣 王喜萍 |
| |
| 作者单位： | 1. 复旦大学生命科学学院遗传工程国家重点实验室,生物多样性与生态工程教育部重点实验室,生物化学系,上海,200433;重庆三峡学院生物系,重庆,404000
2. 复旦大学生命科学学院遗传工程国家重点实验室,生物多样性与生态工程教育部重点实验室,生物化学系,上海,200433 |
| |
| 基金项目： | 中国科学院资助项目;同济大学校科研和教改项目 |
| |
| 摘 要： | 利用Blast检索、EST分析和RT-PCR,在水稻中分离到一个与拟南芥COI1同源的新基因,命名为OsCOI1.OsCOI1编码595个氨基酸.推测的OsCOI1编码蛋白有一个F-boxmotif和16个富含亮氨酸的重复序列,这与拟南芥COI1相似.OsCOI1在氨基酸水平上和COI1有很高的同源性(74%).经半定量RT-PCR法和RNA印迹分析,表明水稻中OsCOI1表达水平在经茉莉酸甲酯和脱落酸处理后呈明显变化,但不受水杨酸和乙烯的影响,说明OsCOI1可能在茉莉酸信号途径和脱落酸途径中具有特定功能.
|
| 关 键 词： | 水稻 F-boxmotif 半定量RT-PCR RNA印迹 茉莉酸信号途径 脱落酸途径 |
OsCOI1, a Putative COI1 in Rice, Show MeJA and ABA Dependent Expression |
| |
| HU Ting-Zhang,WANG Wei-Ping,CAO Kai-Ming,WANG Xi-Ping.OsCOI1, a Putative COI1 in Rice, Show MeJA and ABA Dependent Expression[J].Progress In Biochemistry and Biophysics,2006,33(4). |
| |
| Authors: | HU Ting-Zhang WANG Wei-Ping CAO Kai-Ming WANG Xi-Ping |
| |
| Abstract: | A novel gene, which was a homologue of Arabidopsis COI1 was isolated from rice (Oryza sativa L.) by RT-PCR and designated as OsCOI1. It encoded a protein of 595 amino acids. The similar F-box motif and 16 leucine-rich repeats were found in the deduced protein OsCOI1. OsCOI1 and COI1 showed high homology (74%) at amino acid level. Semi-quantitative RT-PCR and Northern blot analysis demonstrated that the expression of OsCOI1 in rice varied obviously after treatment with MeJA and ABA but was not affected by SA and ET, suggesting that the specific function of OsCOI1 in JA signal pathway and related ABA pathway. |
| |
| Keywords: | rice F-box motif semi-quantitative RT-PCR Northern blot JA signal pathway ABA pathway |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
