| 溶液流动对等电溶菌酶晶体生长的影响 |
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| 引用本文： | 刘山,黄慆,肖雯,周尧和.溶液流动对等电溶菌酶晶体生长的影响[J].生物化学与生物物理进展,1991,18(5):361-364. |
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| 作者姓名： | 刘山 黄慆 肖雯 周尧和 |
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| 作者单位： | 西北工业大学凝固技术国家实验室;西北工业大学凝固技术国家实验室;西北工业大学凝固技术国家实验室;西北工业大学凝固技术国家实验室 |
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| 摘 要： | 本文对等温自由生长和强制性溶液生长的等电溶菌酶的晶体形态进行了研究,发现这些形态变化与溶液相的流动密切相关,指出生物晶体生长停止是由于生长晶体周围的溶质贫乏造成的;通过某些手段减薄或消除这一溶质贫乏区,就可以保证晶体的持续生长。本文的研究对改善大尺寸晶体的生长提供了一条途径。
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| 关 键 词： | 蛋清溶菌酶 溶液流动 生长停止 强制性溶液生长 大尺寸晶体 |
| 收稿时间： | 9/7/1990 12:00:00 AM |
| 修稿时间： | 1990/11/17 0:00:00 |
EFFECTS OF SOLUTION FLOW ON THE GROWTH MORPHOLOGIES OF EGG-WHITE LYSOZYME CRYSTALS AT ISOELECTRIC POINT |
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| Liu Shan,Huang Tao,Xiao Wen and Zhou Yaohe.EFFECTS OF SOLUTION FLOW ON THE GROWTH MORPHOLOGIES OF EGG-WHITE LYSOZYME CRYSTALS AT ISOELECTRIC POINT[J].Progress In Biochemistry and Biophysics,1991,18(5):361-364. |
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| Authors: | Liu Shan Huang Tao Xiao Wen and Zhou Yaohe |
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| Institution: | National lab of solidification processing, northwestern polytechnical university;National lab of solidification processing, northwestern polytechnical university;National lab of solidification processing, northwestern polytechnical university;National lab of solidification processing, northwestern polytechnical university |
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| Abstract: | A careful study has been made on different morphologies of lysozyme crystals at isoelectric point under isothermal and constrained growth conditions. It is found that the morphologies are closely related to solution flow. Judging by the experimental results, the cessation of biological crystal growth is due to the solute-depletion zone stably existing around the growing crystals. Reducing or removing the solute-depletion zone may ensure the continuous crystal growth. This approach throws some light on the possibility of growing largedimensional biological crystals. |
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| Keywords: | egg-white lysozyme solution flow cessation of crystal growth constrained solution growth large-dimensional crystals |
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