| 染色质免疫沉淀-测序：全基因组范围研究蛋白质-DNA相互作用的新技术 |
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| 引用本文： | 梁芳,徐柯,龚朝建,李俏,马健,熊炜,曾朝阳,李桂源.染色质免疫沉淀-测序：全基因组范围研究蛋白质-DNA相互作用的新技术[J].生物化学与生物物理进展,2013,40(3):216-227. |
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| 作者姓名： | 梁芳 徐柯 龚朝建 李俏 马健 熊炜 曾朝阳 李桂源 |
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| 作者单位： | 中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078; 中南大学疾病基因组研究中心湖南省非可控性炎症与肿瘤重点实验室，长沙 410013,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078; 中南大学疾病基因组研究中心湖南省非可控性炎症与肿瘤重点实验室，长沙 410013,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078,中南大学肿瘤研究所，卫生部癌变原理重点实验室及教育部癌变与侵袭原理重点实验室，长沙 410078; 中南大学疾病基因组研究中心湖南省非可控性炎症与肿瘤重点实验室，长沙 410013 |
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| 基金项目： | 国家自然科学基金资助项目(81272298, 30871282, 30871365, 81172189, 81171930)，湖南省自然科学基金(10JJ7003)，霍英东高校青年教师基金(121036)，中央高校基本科研业务费专项资金(2011JQ020)，中南大学米塔尔创新创业项目(11MX27)和中南大学贵重仪器设备开放共享基金资助项目 |
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| 摘 要： | 染色质免疫沉淀-测序(ChIP-seq)是近年来新兴的将染色质免疫沉淀(ChIP)与深度测序技术相结合，在全基因组范围内分析DNA结合蛋白结合位点、组蛋白修饰、核小体定位和DNA甲基化的高通量技术．在新一代测序(NGS)技术的大力推动下，ChIP-seq提供了一种相对于ChIP-chip高分辨率、低噪音、高覆盖率的研究方法．随着测序成本的降低，ChIP-seq逐步成为研究基因调控和表观遗传机制的一种常用手段．本文就该技术的最近研究进展进行综述，并着重介绍ChIP-seq数据分析过程及该技术的实际应用情况.
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| 关 键 词： | ChIP-seq，新一代测序技术，基因调控，表观遗传学，数据分析 |
| 收稿时间： | 2012/6/21 0:00:00 |
| 修稿时间： | 2012/11/21 0:00:00 |
ChIP-seq: a New Technique for Genome-wide Profiling of Protein-DNA Interaction |
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| LIANG Fang,XU Ke,GONG Zhao-Jian,LI Qiao,MA Jian,XIONG Wei,ZENG Zhao-Yang and LI Gui-Yuan.ChIP-seq: a New Technique for Genome-wide Profiling of Protein-DNA Interaction[J].Progress In Biochemistry and Biophysics,2013,40(3):216-227. |
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| Authors: | LIANG Fang XU Ke GONG Zhao-Jian LI Qiao MA Jian XIONG Wei ZENG Zhao-Yang and LI Gui-Yuan |
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| Institution: | Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China,Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, Central South University, Changsha 410013, China,Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, Central South University, Changsha 410013, China,Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China and Key Laboratory of Carcinogenesis of Ministry of Health, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China; Hunan Key Laboratory of Nonresolving Inflammation and Cancer, Disease Genome Research Center, Central South University, Changsha 410013, China |
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| Abstract: | |
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| Keywords: | ChIP-seq next generation sequencing gene regulation epigenetics data analysis |
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