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表达序列标签数据库搜索鉴定小鼠UBAP1基因及其数字化表达分析
引用本文:钱骏,董利,张必成,王洁如,周鸣,李忠花,李伟芳,李小玲,李桂源.表达序列标签数据库搜索鉴定小鼠UBAP1基因及其数字化表达分析[J].生物化学与生物物理进展,2002,29(2):323-327.
作者姓名:钱骏  董利  张必成  王洁如  周鸣  李忠花  李伟芳  李小玲  李桂源
作者单位:中南大学湘雅医学院肿瘤研究所,教育部癌变与侵袭原理重点实验室,长沙,410078
基金项目:国家高技术“863”计划资助项目(102-10-01-05,Z19-01-01-03)、国家重点基础研究发展规划项目(973)(G1998051008)及美国中华医学基金资助(96655).
摘    要:UBAP1(ubiquitin associated protein 1)基因是最近克隆的一个定位于人类染色体9p21-22鼻咽癌杂合性丢失高频区的泛肽相关蛋白家族新成员.为了深入研究UBAP1基因的功能,利用计算机对表达序列标签(expressed sequence tag, EST)、UniGene等数据库进行综合搜索分析,结合cDNA克隆测序的方法, 成功地获得了UBAP1基因在小鼠中的同源基因.小鼠UBAP1基因cDNA全长为2 676 bp,编码一个由441个氨基酸组成的蛋白质,在其蛋白质C端只有一个泛肽相关功能域(UBA domain).与人UBAP1基因相比,两者编码的氨基酸序列有89%相同.基于EST的数字化表达分析显示UBAP1基因在小鼠正常组织中广泛高表达.

关 键 词:UBAP1基因,基因克隆,表达序列标签,组织表达
收稿时间:2001/6/27 0:00:00
修稿时间:2001年6月27日

Identification and Digitalized Expression Analysis of Murine UBAP1 Gene by Means of EST Database Searching
QIAN Jun,DONG Li,ZHANG Bi-Cheng,WANG Jie-Ru,ZHOU Ming,LI Zhong-Hu,LI Wei-Fang,LI Xiao-Ling and LI Gui-Yuan.Identification and Digitalized Expression Analysis of Murine UBAP1 Gene by Means of EST Database Searching[J].Progress In Biochemistry and Biophysics,2002,29(2):323-327.
Authors:QIAN Jun  DONG Li  ZHANG Bi-Cheng  WANG Jie-Ru  ZHOU Ming  LI Zhong-Hu  LI Wei-Fang  LI Xiao-Ling and LI Gui-Yuan
Institution:Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China;Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory of Carcinogenesis, Chinese Ministry of Education, Changsha 410078, China
Abstract:Ubiquitin associated protein 1(UBAP1) is a novel member of UBA domain protein family, located at human chromosome 9p21-22 where loss of heterozygosity frequently occurs in nasopharyngeal carcinoma. Through integrated analysis of public database such as EST, Unigene in silico and direct sequencing of cDNA clone, full-length cDNA sequence of murine homologoue of human UBAP1 gene was identified, which is 2 676 bp long and encodes a putative protein of 441 amino acids with a UBA domain. There is an average of 89% identical residues between mouse and human UBAP1 protein. Digitalized expression analysis based on EST data showed that mouse UBAP1 gene expressed ubiquitiously and strongly in most of mouse normal tissues.
Keywords:UBAP1  gene cloning  expressed sequence tag(EST)  tissue expression
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