| Visualization of Mitochondrial Membrane Dynamics in Primary Cultured Neurons by Cryo-electron Tomography |
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| 作者姓名： | 王 培 田步云 冯凤萍 栾会芹 徐晓君 纪 伟 薛艳红 |
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| 作者单位： | 1)中国科学院生物物理研究所，中国科学院生物大分子卓越创新中心，生物大分子国家重点实验室，北京，100101;2)中国科学院大学，北京，100049,1)中国科学院生物物理研究所，中国科学院生物大分子卓越创新中心，生物大分子国家重点实验室，北京，100101;2)中国科学院大学，北京，100049,1)中国科学院生物物理研究所，中国科学院生物大分子卓越创新中心，生物大分子国家重点实验室，北京，100101,3)国家康复辅具研究中心，北京，100176,1)中国科学院生物物理研究所，中国科学院生物大分子卓越创新中心，生物大分子国家重点实验室，北京，100101,1)中国科学院生物物理研究所，中国科学院生物大分子卓越创新中心，生物大分子国家重点实验室，北京，100101;2)中国科学院大学，北京，100049,1)中国科学院生物物理研究所，中国科学院生物大分子卓越创新中心，生物大分子国家重点实验室，北京，100101 |
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| 基金项目： | 国家重点研发计划（2017YFA0504700）和国家自然科学基金（32027901）资助项目. |
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| 摘 要： | 本文利用冷冻电子断层扫描成像技术研究了原代培养海马神经元中线粒体膜的动态变化. 线粒体的分裂与融合是线粒体膜动态变化的主要方式，也是维持线粒体功能正常的重要手段. 线粒体分裂的机制研究以往是基于荧光标记的光学显微成像，由于分辨率的限制并不能直接观察到线粒体分裂过程中的超微结构特征. 冷冻电子断层成像通过尽可能保持样品生理状态从而获得更真实的结构信息. 本文通过对原代海马神经元中的自发性线粒体膜动态变化的成像，发现中央分裂和外周分裂的线粒体都与内质网在空间上存在一定的相互作用，内质网通过缠绕在线粒体分裂位点来参与分裂过程. 值得注意的是，还发现部分线粒体会出现线粒体外膜与内膜分离的现象，形成“无基质”的特殊区域. 这些可能都表明了线粒体质量控制的方式.
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| 关 键 词： | 冷冻电子断层扫描 海马神经元 线粒体分裂 |
| 收稿时间： | 2021/10/7 0:00:00 |
| 修稿时间： | 2021/11/3 0:00:00 |
Visualization of Mitochondrial Membrane Dynamics in Primary Cultured Neurons by Cryo-electron Tomography |
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| WANG Pei,TIAN Bu-Yun,FENG Feng-Ping,LUAN Hui-Qin,XU Xiao-Jun,JI Wei and XUE Yan-Hong.Visualization of Mitochondrial Membrane Dynamics in Primary Cultured Neurons by Cryo-electron Tomography[J].Progress In Biochemistry and Biophysics,2021,48(11):1365-1368. |
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| Authors: | WANG Pei TIAN Bu-Yun FENG Feng-Ping LUAN Hui-Qin XU Xiao-Jun JI Wei and XUE Yan-Hong |
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| Institution: | 1)National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 2)University of Chinese Academy of Sciences, Beijing 100049, China,1)National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 2)University of Chinese Academy of Sciences, Beijing 100049, China,1)National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China,3)National Research Center for Rehabilitation Technical Aids, Beijing 100176, China,1)National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China,1)National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 2)University of Chinese Academy of Sciences, Beijing 100049, China,1)National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | The fission and fusion of mitochondria is the main way of dynamic changes of mitochondrial membrane, and it is also an important means to maintain the normal function of mitochondria. The mechanism of mitochondrial fission used to be based on fluorescent-labeled optical microscopy imaging. Due to the limitation of resolution, it is not possible to directly observe the ultrastructural characteristics of the mitochondrial fission. Cryo-electron tomography (cryo-ET) obtains more realistic structural information by maintaining the physiological state of the sample as much as possible. In this article, we use cryo-ET to study the membrane dynamic of mitochondrial fission in primary hippocampal neurons. By imaging the spontaneous mitochondrial fission, we found that both central and peripheral mitochondrial fission have contact with the endoplasmic reticulum (ER). It is worth noting that we have also found that some mitochondria will separate the outer mitochondrial membrane from the inner membrane, forming a "matrix-free" area. All these may show a way of mitochondrial quality control. |
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| Keywords: | Cryo-electron tomography hippocampal neuron mitochondrial fission |
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