一种新的抑癌候选基因——NDR2在人类正常组织及相应肿瘤中的表达

为观察NDR2基因在人类正常组织及相应肿瘤组织中的表达分布特点，收集人脑与胶质瘤、肺与肺癌、胃与胃癌、结肠与结肠癌组织标本，分别进行组织石蜡切片和总RNA提取，应用免疫组化方法和RT-PCR技术检测NDR2蛋白质及mRNA表达水平，并通过DNA测序验证PCR产物的正确性.免疫组化结果表明，在上述各组织均有NDR2蛋白不同程度的表达.RT-PCR结果显示，在人脑和胶质瘤组织、肺与肺癌组织、胃与胃癌组织、结肠与结肠癌组织中均有NDR2 mRNA表达，其表达水平以脑组织为最高.NDR2 mRNA在正常脑和肺组织的表达水平分别显著高于胶质瘤与肺癌组织，而结肠与结肠癌组织，胃与胃癌组织NDR2 mRNA表达水平则无显著差别.以上结果表明，NDR2基因可能广泛表达于人体正常组织内，而在胶质瘤与肺癌中的表达较相应正常组织减低，提示该基因可能与神经系统及呼吸系统肿瘤的发生发展有关，为进一步探讨该基因功能提供了线索.

Expression Pattern of NDR2 Gene, A Candidate Tumor-suppressor, in Different Human Tissues and Tumors

In order to further probe the function of NDR2 gene, the expression of NDR2 gene was determined in different human tissues and their relevant tumors. Tissues of human brain and glioma, lung and lung cancer, colon and carcinoma of colon, stomach and carcinoma of stomach were collected for total RNA extraction and paraffin-embedment. Immunohistochemistry and RT-PCR were used to examine the expression of NDR2 both in mRNA and protein level. DNA sequencing was performed to confirm the result of RT-PCR. Immunohistochemistry results showed that NDR2 protein was extensively expressed in the tissues. RT-PCR further showed an extensive expression of NDR2 mRNA, with a higher expression level in normal brain and lung tissue than glioma and lung cancer respectively, while no differences were observed between colon and carcinoma of colon, stomach and carcinoma of stomach. These results suggest a possible involvement of NDR2 in the genesis and progression of glioma and lung cancer, which provide a clue for further studies on NDR2.