| 不同来源乳铁蛋白对幼鼠肠道发育的影响 |
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| 引用本文： | 程智美,王文利,李泽阳,孟庆勇,戴蕴平,张雅丽.不同来源乳铁蛋白对幼鼠肠道发育的影响[J].生物化学与生物物理进展,2022,49(8):1555-1563. |
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| 作者姓名： | 程智美 王文利 李泽阳 孟庆勇 戴蕴平 张雅丽 |
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| 作者单位： | 1）中国农业大学食品科学与营养工程学院,北京 100083,1）中国农业大学食品科学与营养工程学院,北京 100083,1）中国农业大学食品科学与营养工程学院,北京 100083,2）中国农业大学生物学院,北京 100193,2）中国农业大学生物学院,北京 100193,1）中国农业大学食品科学与营养工程学院,北京 100083 |
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| 基金项目： | 财政部和农业农村部国家现代农业产业技术体系和农业农村部转基因重大专项（2018ZX08007001）资助。 |
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| 摘 要： | 目的 探究哺乳期乳铁蛋白（lactoferrin,LF）的缺失及不同来源LF补充后对幼鼠肠道发育的影响。方法 以LF基因敲除型雌鼠作为哺乳母鼠造成幼鼠哺乳期无LF的摄入,且从幼鼠出生第3~21天每日人工饲喂100 mg/kg 牛血清白蛋白（BSA）、牛源乳铁蛋白（bovine Lactoferrin,bLF）及重组人源乳铁蛋白（recombinant human Lactoferrin,rhLF）,于幼鼠21日龄取样,测定各组小鼠小肠发育指标。结果 在本实验周期下,哺乳期rhLF的补充显著性增加小鼠回肠绒毛长度/隐窝深度值（P<0.05）,且上调回肠Occludin和ZO-1基因的表达（P<0.05）,增加小鼠十二指肠、空肠和回肠麦芽糖酶酶活/乳糖酶酶活比值（P<0.05）,表明哺乳期rhLF的补充能够增强小鼠肠道消化吸收能力和肠屏障功能;哺乳期bLF的补充显著增加小鼠十二指肠及回肠麦芽糖酶活性/乳糖酶活性比值（P<0.05）。结论 对于哺乳期无LF摄入的乳鼠来说,哺乳期间LF的补充能够增强乳鼠肠道对营养物质的消化吸收能力、促进肠道的发育成熟、增强肠道屏障功能,并且,本实验中rhLF表现出比bLF更加有效的作用。
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| 关 键 词： | 牛乳铁蛋白 重组人源乳铁蛋白 肠道发育 新生乳鼠 |
| 收稿时间： | 2021/8/20 0:00:00 |
| 修稿时间： | 2022/7/24 0:00:00 |
Effects of Lactoferrin From Different Sources on Intestinal Development in Newborn Mice |
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| CHENG Zhi-Mei,WANG Wen-Li,LI Ze-Yang,MENG Qing-Yong,DAI Yun-Ping and ZHANG Ya-Li.Effects of Lactoferrin From Different Sources on Intestinal Development in Newborn Mice[J].Progress In Biochemistry and Biophysics,2022,49(8):1555-1563. |
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| Authors: | CHENG Zhi-Mei WANG Wen-Li LI Ze-Yang MENG Qing-Yong DAI Yun-Ping and ZHANG Ya-Li |
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| Institution: | 1)College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China,1)College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China,1)College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China,2)College of Biological Science, China Agricultural University, Beijing 100193, China,2)College of Biological Science, China Agricultural University, Beijing 100193, China,1)College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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| Abstract: | Objective To investigate the effects of lactoferrin (LF) deficiency during lactation and LF supplementation from different sources on intestinal development of newborn mice.Methods Female LF knockout mice were used to produce LF deficiency milk for newborn mice intake during lactation, and 100 mg/kg bovine Lactoferrin (bLF), recombinant human Lactoferrin (rhLF) and bovine serum albumin (BSA) were artificially fed every day from the 3rd day to the 21st day. Samples were taken at the age of 21 days to determine the small intestine development index of mice in each group.Results In this experimental cycle, rhLF supplementation significantly increased the villus length/crypt depth of ileum (P<0.05), up-regulated the expression of Occludin and ZO-1 in ileum (P<0.05) and increased the ratio of maltase activity/lactase activity in duodenum (P<0.05), jejunum (P<0.05) and ileum (P<0.05) of mice. In addition, the supplementation of bLF only significantly increased the ratio of maltase activity/lactase activity in ileum (P<0.05), indicating that the supplementation of LF during lactation can promote the intestinal development of newborn mice.Conclusion For newborn mouse without LF intake during lactation, the supplement of LF during lactation can enhance the intestinal digestion and absorption of nutrients, promote the development and maturity of the intestinal tract, and enhance the intestinal barrier function. Moreover, rhLF plays a more effective role than bLF. |
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| Keywords: | bovine lactoferrin recombinant human lactoferrin intestinal development newborn mouse |
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