| RASSF1A对黑色素瘤A375细胞基因网络的影响 |
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| 引用本文： | 易梅,李吉,粟娟,张江林,陈翔,杜乾君,向波,熊炜,李小玲,谢红付.RASSF1A对黑色素瘤A375细胞基因网络的影响[J].生物化学与生物物理进展,2014,41(4):352-361. |
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| 作者姓名： | 易梅 李吉 粟娟 张江林 陈翔 杜乾君 向波 熊炜 李小玲 谢红付 |
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| 作者单位： | 中南大学湘雅医院皮肤性病科，长沙 410008；中南大学肿瘤研究所，长沙 410078,中南大学湘雅医院皮肤性病科，长沙 410008,中南大学湘雅医院皮肤性病科，长沙 410008,中南大学湘雅医院皮肤性病科，长沙 410008,中南大学湘雅医院皮肤性病科，长沙 410008,中南大学湘雅医院皮肤性病科，长沙 410008,中南大学肿瘤研究所，长沙 410078；中南大学湘雅医学院附属肿瘤医院(湖南省肿瘤医院)，长沙 410013,中南大学肿瘤研究所，长沙 410078；中南大学湘雅医学院附属肿瘤医院(湖南省肿瘤医院)，长沙 410013,中南大学肿瘤研究所，长沙 410078；中南大学湘雅医学院附属肿瘤医院(湖南省肿瘤医院)，长沙 410013,中南大学湘雅医院皮肤性病科，长沙 410008 |
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| 基金项目： | 国家自然科学基金(81102065，81171930)，教育部高等学校博士学科点基金(20110162120009)，中南大学自由探索计划(201012200017，2011QNZT138)资助项目 |
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| 摘 要： | RASSF1A(Ras association domain family 1 isoform A)是定位于染色体3p21.3区域的抑瘤基因,编码一个由340个氨基酸残基构成的微管相关蛋白.该基因在包括恶性黑色素瘤在内的多种肿瘤中因启动子高甲基化而表达沉默.本研究建立了RASSF1A稳定表达的恶性黑色素瘤A375细胞系,通过全基因组表达谱基因芯片分析RASSF1A过表达对A375细胞基因表达谱的影响,发现RASSF1A引起184个基因表达上调,26个基因表达下调.通过Realtime RT-PCR对部分差异表达基因进行验证,结果表明与芯片筛选结果一致.RASSF1A影响的差异表达基因功能上归属于细胞生长与增殖、细胞周期、细胞凋亡、细胞间黏附、信号传导等生物过程.采用STRING软件构建了RASSF1A影响的差异表达基因调控网络,结果表明RASSF1A调控的差异表达基因构成一个高连接度的基因网络.其中,炎症细胞因子、转录因子位于网络中央.RASSF1A通过影响炎症细胞因子与转录因子之间的表达,影响A375细胞基因网络,调节黑色素瘤恶性生物学行为.
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| 关 键 词： | RASSFA 恶性黑色素瘤 基因微阵列 基因调控网络 |
| 收稿时间： | 6/6/2013 12:00:00 AM |
| 修稿时间： | 2013/8/20 0:00:00 |
Gene Network Modulated by RASSF1A Over-expression in Melanoma A375 Cells |
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| YI Mei,LI Ji,SU Juan,ZHANG Jiang-Lin,CHEN Xiang,DU Qian-Jun,XIANG Bo,XIONG Wei,LI Xiao-Ling and XIE Hong-Fu.Gene Network Modulated by RASSF1A Over-expression in Melanoma A375 Cells[J].Progress In Biochemistry and Biophysics,2014,41(4):352-361. |
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| Authors: | YI Mei LI Ji SU Juan ZHANG Jiang-Lin CHEN Xiang DU Qian-Jun XIANG Bo XIONG Wei LI Xiao-Ling and XIE Hong-Fu |
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| Institution: | Department of Dermatology, Xiangya Hospital of Central South University, Changsha 410078, China;Cancer Research Institute of Central South University, Changsha 410078, China,Department of Dermatology, Xiangya Hospital of Central South University, Changsha 410078, China,Department of Dermatology, Xiangya Hospital of Central South University, Changsha 410078, China,Department of Dermatology, Xiangya Hospital of Central South University, Changsha 410078, China,Department of Dermatology, Xiangya Hospital of Central South University, Changsha 410078, China,Department of Dermatology, Xiangya Hospital of Central South University, Changsha 410078, China,Cancer Research Institute of Central South University, Changsha 410078, China;Hunan Provinvial Tumor Hospital and The Tumor Hospital Affiliated to Xiongya School of Medicine,Central South University, Changsha 410013, China,Cancer Research Institute of Central South University, Changsha 410078, China;Hunan Provinvial Tumor Hospital and The Tumor Hospital Affiliated to Xiongya School of Medicine,Central South University, Changsha 410013, China,Cancer Research Institute of Central South University, Changsha 410078, China;Hunan Provinvial Tumor Hospital and The Tumor Hospital Affiliated to Xiongya School of Medicine,Central South University, Changsha 410013, China and Department of Dermatology, Xiangya Hospital of Central South University, Changsha 410078, China |
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| Abstract: | |
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| Keywords: | RASSF1A malignant melanoma microarray gene regulatory network |
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