荧光标记的染色体原位杂交及其在人类基因组研究中的应用

FISH技术是80年代开始发展起来的一种新的定位技术.在人类基因组研究中得到了广泛的应用.通过中期染色体的FISH可以进行SCP,Cosmid和YAC的染色体定位,嵌合克隆的鉴别;通过间期核的FISH可以在50kb的分辨率下进行基因作图;最新的研究进展已可以进行伸展的染色质丝(chromatin fibre)的FISH,直接测量基因的长度,从而达到高精度基因作图的目的.总之,随着FISH技术本身的发展,它将在人类基因组研究中发挥更大的作用.

FISH and its Application in Human Genome Research

FISH is a new technique which is used to localize a specific sequence on chromosome,It has been widely used in the research of human genome project.With FISH,a SCP,a cosmid or a YAC can be localized on the metaphase chromosome,and a chimeric YAC can also be identified.Gene mapping can be done at the resolution of 50 kb with FISH on the interphase nuclei.Now FISH of a gene on the strechted chromatic fibre can be done,the length of the gene can directly be measured and the gene can be localized at high resolution. In a word. FISH is playing a more and more important part in the human genome research.