| 亲和层析纯化肌质网Ca2+－ATP酶 |
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| 引用本文： | 屠亚平,徐红.亲和层析纯化肌质网Ca2+－ATP酶[J].生物化学与生物物理进展,1995,22(4):345-349. |
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| 作者姓名： | 屠亚平 徐红 |
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| 作者单位： | 中国科学院生物物理研究所生物大分子国家重点实验室;中国科学院生物物理研究所生物大分子国家重点实验室 |
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| 摘 要： | 建立了一种亲和层析纯化肌质网Ca2+-ATP酶的方法．用非离子型去污剂C12E8 溶解肌质网，再通过反应红－120琼脂糖亲和层析柱使肌质网Ca2+-ATP酶纯度从粗品中的65％提高到99％，并具有较高ATP水解活性．经SDS－聚丙烯酰胺凝胶电泳检测，为电泳纯．
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| 关 键 词： | 肌质网Ca2+－ATP酶 反应红－120琼脂糖 亲和层析 |
| 收稿时间： | 1995/1/12 0:00:00 |
| 修稿时间： | 1995/2/15 0:00:00 |
Purification of Ca2+-ATPase from Sarcoplasmic Reticulum by Affinity Gel Chromatography |
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| Tu Yaping and Xu Hong.Purification of Ca2+-ATPase from Sarcoplasmic Reticulum by Affinity Gel Chromatography[J].Progress In Biochemistry and Biophysics,1995,22(4):345-349. |
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| Authors: | Tu Yaping and Xu Hong |
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| Institution: | National Key Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica;National Key Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica |
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| Abstract: | Ca2+-ATPase from rabbit skeletal muscle sarcoplasmic reticulum vesicles was solubilized by nonionic detergent C12E8 and purified in a reactive red-120 agrose affinity column.Determined by 7.5% SDS-PAGE,the purity of Ca2+-ATPase was increased from 65% to 99% and the purified Ca2+-ATPase exhibited higher ATP hydrolysis activity. |
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| Keywords: | sarcoplasmic reticulum Ca2+-ATPase reactive red-120 agrose affinity gel filtration |
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