| 微型离心柱法快速分离纯化神经节苷脂 |
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| 引用本文： | 王江雁,张卫宁,王仁杰,许玉茹,王梦寅.微型离心柱法快速分离纯化神经节苷脂[J].生物化学与生物物理进展,1997,24(1):75-78. |
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| 作者姓名： | 王江雁 张卫宁 王仁杰 许玉茹 王梦寅 |
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| 作者单位： | 解放军石家庄医学高等专科学校生化教研室，石家庄 050081;解放军石家庄医学高等专科学校生化教研室，石家庄 050081;解放军石家庄医学高等专科学校生化教研室，石家庄 050081;解放军石家庄医学高等专科学校生化教研室，石家庄 050081;解放军石家庄医学高等专科学校生化教研室，石家庄 050081 |
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| 摘 要： | 神经节苷脂的分离与纯化是研究组织细胞Gls组成、含量及代谢的基本手段.但是以往的方法周期长、溶剂用量大，为此建立了一种新的微型离心柱快速分离Gls纯化法，标准Gls的回收率为97.64％，ｎ＝7，ＣＶ＜３％，HPTLC图谱显示无丢带现象.纯化样品只需十几个小时，操作简单，溶剂用量小，重复性好，为微量样品组分的研究提供了一个有效的手段.
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| 关 键 词： | 微型离心柱 分离 纯化 神经节苷脂 |
| 收稿时间： | 2/8/1996 12:00:00 AM |
| 修稿时间： | 1996/7/17 0:00:00 |
Isolation and Purification of Gangliosides by Rapid Centrifugal Mini-column Chromatography |
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| WANG Jiangyan,ZHANG Weining,WANG Renjie,XU Yuru and WANG Mengyin.Isolation and Purification of Gangliosides by Rapid Centrifugal Mini-column Chromatography[J].Progress In Biochemistry and Biophysics,1997,24(1):75-78. |
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| Authors: | WANG Jiangyan ZHANG Weining WANG Renjie XU Yuru and WANG Mengyin |
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| Institution: | Department of Biochemistry, Shijiazhuang Medical Higher Training School, Shijiazhuang 050081, China;Department of Biochemistry, Shijiazhuang Medical Higher Training School, Shijiazhuang 050081, China;Department of Biochemistry, Shijiazhuang Medical Higher Training School, Shijiazhuang 050081, China;Department of Biochemistry, Shijiazhuang Medical Higher Training School, Shijiazhuang 050081, China;Department of Biochemistry, Shijiazhuang Medical Higher Training School, Shijiazhuang 050081, China |
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| Abstract: | Asimpleandrapidmethodforisola tionandpurificationofgangliosides (Gls)from theratbrainisdescribed .Thegangliosideswere isolatedandpurifiedbycentrifugalmini column chromatography.Therateofrecoveryofstan dardGlswasupto 97 6 4% ,n =7,CV <3% . NodetectivelossofGlswasdemonstrated .The novelmethodoffersafewadvantages:1 Itis simple,rapidandefficient.2 Thethinlayer chromatographicpatternsoftotalGlsofsamples obtainedareclearanddistinct.3 Lesssolvent wasconsumed .Thus,themethodisespecially applicabletocomparativeandqualitativestudies ofgangliosidesofmicrosamples,andtoganlio sidemetabolismresearchoftissuesandcellsin vitro. |
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| Keywords: | centrifugal mini-column chromatography isolation purification gangliosides |
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