| 一种SDS-PAGE与MALDI-TOF质谱联用的方法 |
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| 引用本文： | 潘晓宇,赵晖,胡美浩.一种SDS-PAGE与MALDI-TOF质谱联用的方法[J].生物化学与生物物理进展,1999,26(4):393-396. |
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| 作者姓名： | 潘晓宇 赵晖 胡美浩 |
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| 作者单位： | 北京大学生命科学学院,蛋白质工程和植物基因工程国家重点实验室,北京,100871 |
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| 基金项目： | 北京大学与美国惠普公司实验室合作项目资助. |
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| 摘 要： | 以尿激酶原为材料，探索一种从SDS-PAGE胶上回收蛋白质做MALDI-TOF质谱的方法.所用的回收方法包括电洗脱、脱盐、除SDS等过程.电洗脱用的是高盐阻断法，脱盐用的是超滤技术，去除SDS用的是冷丙酮沉淀法.结果证明，此方法至少对一些蛋白质（如尿激酶原和牛血清白蛋白）是可行的.
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| 关 键 词： | 尿激酶原，SDS-聚丙烯酰胺凝胶电泳，基质辅助激光解吸附/离子化飞行时间质谱 |
| 修稿时间： | : 1998-05- |
A Method of Recovering Protein from Dodecyl Sulfate-polyacrylamide Gel for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry |
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| PAN Xiao-Yu,ZHAO Hui,HU Mei-Hao.A Method of Recovering Protein from Dodecyl Sulfate-polyacrylamide Gel for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry[J].Progress In Biochemistry and Biophysics,1999,26(4):393-396. |
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| Authors: | PAN Xiao-Yu ZHAO Hui HU Mei-Hao |
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| Institution: | National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Beijing University, Beijing 100871, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Beijing University, Beijing 100871, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Beijing University, Beijing 100871, China |
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| Abstract: | The purpose of the experiment is to use pro-urokinase as a model to find a way of recovering proteins from SDS-PAGE gel for MALDI-TOF MS. The recovering method includes steps of electroelution, desalting and SDS removal. Discontinuous conductivity gradient method for electroelution, centrifugation ultrofiltration for desalting and cold acetone method was used for SDS removal. The result shows that the method is feasible at least for some proteins (e.g.pro-urokinase and bovine serum albumin). |
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| Keywords: | pro-urokinase SDS-PAGE matrix-assisted laser desorption/ionization mass spectrometry(MALDI-TOF) |
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