| AP-1辅助激活因子JAB1的分子克隆及其与肝细胞生成素的相互作用 |
| |
| 引用本文： | 陆承荣,李勇,董春娜,黎明,王清明,张成岗,魏玉琳,陈惠鹏,贺福初.AP-1辅助激活因子JAB1的分子克隆及其与肝细胞生成素的相互作用[J].生物化学与生物物理进展,2001,28(4):528-531. |
| |
| 作者姓名： | 陆承荣 李勇 董春娜 黎明 王清明 张成岗 魏玉琳 陈惠鹏 贺福初 |
| |
| 作者单位： | 军事医学科学院放射医学研究所, |
| |
| 基金项目： | 国家自然科学基金重点项目(39830440)、国家重点基础研究项目（G1998051122）及国家自然科学基金项目（30070171）部分资助. |
| |
| 摘 要： | 利用酵母双杂交方法，用肝细胞生成素(HPO)作为诱饵蛋白在人胎肝cDNA文库中筛选到能与HPO相互作用的蛋白：AP-1辅助激活因子JAB1.并用PCR方法从人胎肝cDNA文库中扩出JAB1全长cDNA，进行GST-JAB1原核融合蛋白表达与纯化.蛋白质结合实验表明，JAB1与人重组HPO以及COS7真核表达的HPO在体外有结合作用.
|
| 关 键 词： | 肝细胞生成素 c-Jun结合蛋白 转录因子 |
| 收稿时间： | 2000/7/19 0:00:00 |
| 修稿时间： | 2000年7月19日 |
Cloning of AP1 Coactivator JAB1 and Its Association with Hepatopoietin |
| |
| LU Cheng-Rong,LI Yong,DONG Chun-N,LI Ming,WANG Qing-Ming,ZHANG Cheng-Gang,WEI Yu-Lin,CHEN Hui-Peng and HE Fu-Chu.Cloning of AP1 Coactivator JAB1 and Its Association with Hepatopoietin[J].Progress In Biochemistry and Biophysics,2001,28(4):528-531. |
| |
| Authors: | LU Cheng-Rong LI Yong DONG Chun-N LI Ming WANG Qing-Ming ZHANG Cheng-Gang WEI Yu-Lin CHEN Hui-Peng and HE Fu-Chu |
| |
| Institution: | Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China;Beijing Institute of Radiation Medicine, Beijing 100850, China |
| |
| Abstract: | Using hepatopioetin (HPO) as "bait" in an yeast two hybrid screen resulted in the identification of JAB1(a protein initially as a co activator of c Jun) as a putative HPO binding partner.Then the full length cDNA of JAB1 was obtained from human fetal liver cDNA library by PCR for GST JAB1 expression.The binding assay showed that JAB1 binded to rHPO and HPO expressed in COS7 cells in vitro . |
| |
| Keywords: | hepatopoietin c-Jun activating-domain binding protein 1 activator protein 1 |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
