三种藻胆蛋白在复合累积LB膜中的能量传递

制备了R-藻红蛋白(R-PE)、C-藻蓝蛋白(C-PC)及变藻蓝蛋白(APC)的单组分累积LB膜及三组分复合累积LB膜. 吸收及荧光光谱测定表明三种蛋白的LB膜的光谱重叠性质与他们在水溶液中的性质相同. 结构测定表明, 三种蛋白在其LB膜中排列有序, 且由它们组装的复合累积LB膜结构类似于藻类植物中的藻胆体结构. 通过稳态荧光光谱及其光谱解叠, 观察到了三种蛋白在复合累积LB膜中的激发能量传递现象. 根据给体荧光峰的猝灭, 计算出在复合累积LB膜中从给体R-PE经C-PC到受体APC的能量传递效率为51%.

Study of Energy Transfer Among Three Kinds of Biliproteins in Their Stacking Langmuir-Blodgett Multilayers

Single components and multicomponent of R-phycoerythrin (R-PE ), Cphycocyanin (C-PC ) and allophycocyanin(APC) LB multilayers were prepared. The three phycobiliproteins in the LB films were orderly oriented as in the natural phycobilisomes. The absorption and fluorescence spectra remainedsimilarly to these in their aqueous solutions. The phenomenon of excitation energy transfer among R-PE, C-PC and APC in their stacking LB multilayers were observed through steady-state fluorescence spectral measurements and spectral deconvolutions. From the quenching degree of R-PE fluorescence, the efficiency of energy transfer from R-PE through C-PC to APC in their stacking LB multilayers was calculated to be 51%.