| 人细胞差异表达基因cDNA消减杂交体系的建立 |
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| 引用本文： | 黎伯铨,吴旻.人细胞差异表达基因cDNA消减杂交体系的建立[J].生物化学与生物物理进展,1996,23(4):364-369. |
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| 作者姓名： | 黎伯铨 吴旻 |
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| 作者单位： | 中国医学科学院肿瘤研究所, 分子肿瘤学国家重点实验室;中国医学科学院肿瘤研究所, 分子肿瘤学国家重点实验室 |
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| 基金项目： | 国家“863”高科技发展计划、攀登计划，博士后基金，WorldLab．资助项目． |
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| 摘 要： | 为克隆肺腺癌分化相关基因, 采用诱导分化与消减杂交相结合的策略, 建立了全反式维甲酸(RA)诱导前后人肺腺癌细胞系的cDNA消减文库, 得到124个cDNA消减克隆. 经加减法杂交差异筛选、DNA和RNA印迹、cDNA全序列测定和生物学功能分析, 分离到3个在人肺腺癌细胞系分化过程中由RA激活而特异表达的新的cDNA序列这一策略和技术路线适用于分离细胞中呈过量表达或表达抑制基因的cDNA克隆, 并具有反映细胞分化过程中基因表达动态变化特征和相对简便适用的特点.
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| 关 键 词： | 消减杂交 分化相关基因 cDNA克隆 维甲酸 肺癌 |
| 收稿时间： | 1995/9/18 0:00:00 |
| 修稿时间： | 1995/12/5 0:00:00 |
A Strategy for Isolating Differentially Expressed Gene From Human Cells |
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| Li Baiquan and Wu Min.A Strategy for Isolating Differentially Expressed Gene From Human Cells[J].Progress In Biochemistry and Biophysics,1996,23(4):364-369. |
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| Authors: | Li Baiquan and Wu Min |
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| Institution: | National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences;National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences |
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| Abstract: | A subtractive hybridization system for isolating differentiation associated gene from human cancer cell line is developed. By using the strategy of differentiation-inducing in combina tion with cDNA-cDNA subtractive hybridization, a subtracted cDNA library from human lung adenocarcinoma cell line before and after treatment with all-trans retinoic acid(RA) was established. Three cDNAs response to RA treatment were obtained by screening of the subtracted cDNA library. The results of sequencing and function analysis show that three cDNAs are novel cDNAs of differentiation associated genes in human lung adenocarcinoma cell line. This system is also suitable for isolating cDNAs representing deleted or overexpressed gene in human cells. |
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| Keywords: | subtractive hybridization differentiation associated gene cDNA clone retinoic acid lung neoplasm |
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