| 壳聚糖固定化纤维素酶的研究 |
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| 引用本文： | 陈盛,黄智跃,刘艳如.壳聚糖固定化纤维素酶的研究[J].生物化学与生物物理进展,1996,23(3):250-254. |
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| 作者姓名： | 陈盛 黄智跃 刘艳如 |
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| 作者单位： | 福建师范大学高分子研究所;福建师范大学高分子研究所;福建师范大学高分子研究所 |
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| 摘 要： | 以蟹壳为原料提取壳聚糖，用戊二醛作交联剂，将纤维素酶固定于壳聚糖上．同时探讨了一定量干壳聚糖载体与交联剂浓度、给酶量等关系的最适固定化酶条件，并对固定化酶的热稳定性、操作稳定性、米氏常数、最适温度、离子强度的影响及使用半衰期等理化性质进行了探讨．
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| 关 键 词： | 纤维素酶 戊二醛 壳聚糖 固定化 |
| 收稿时间： | 7/5/1995 12:00:00 AM |
| 修稿时间： | 1995/9/21 0:00:00 |
Study on Chitosan-immobilized Cellulase |
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| Chen Sheng,Huang Zhiyue and Liu Yanru.Study on Chitosan-immobilized Cellulase[J].Progress In Biochemistry and Biophysics,1996,23(3):250-254. |
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| Authors: | Chen Sheng Huang Zhiyue and Liu Yanru |
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| Institution: | Institute of Polymer Science, Fujian Normal University;Institute of Polymer Science, Fujian Normal University;Institute of Polymer Science, Fujian Normal University |
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| Abstract: | Chitosan was obtained from crab shells by treating with HCl and NaOH. The conditions of cellulase immobilized on chitosan by glutaraldehyde were studied. The results indicated that the immobilized-cellulase prepared by 0.1g dry chitosan cross-linking with 5% glutaraldhyde and then combining with 4.0 mg cellulase showed higher cellulase activity and better activity recoveries (75% ). And some characters of immobilized and native cellulases, such as optimum temperature Michaelis constant and the effect of ionic strength, stabilities to heat and repeat operation were studied and compared. |
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| Keywords: | chitosan cellulase immobilization glutaraldehyde |
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