| 转基因猪体内外源基因的整合鉴定 |
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| 引用本文： | 刘桂生,陈清轩,王菁,魏庆信,陈永福.转基因猪体内外源基因的整合鉴定[J].生物化学与生物物理进展,1996,23(3):279-280. |
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| 作者姓名： | 刘桂生 陈清轩 王菁 魏庆信 陈永福 |
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| 作者单位： | 中国科学院发育生物学研究所;中国科学院发育生物学研究所;中国科学院发育生物学研究所;湖北省农业科学院畜牧兽医研究所;中国农业大学生物技术学院 |
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| 基金项目： | 国家“863”计划资助项目. |
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| 摘 要： | 外源猪生长激素基因被导入湖北白猪．为避免羊、猪之间在金属硫蛋白启动上的同源性而造成PCR检测结果的假阳性，分别将PCR合成反应的两个引物设计在羊金属硫蛋白基因启动子一侧和猪生长激素一侧，使PCR合成反应的结果特异地反映外源基因的整合性，准确地鉴定出转基因猪．DNA印迹分析结果表明，43头待检仔猪中有6头呈外源基因整合阳性．
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| 关 键 词： | 猪生长激素基因 PCR DNA印迹 转基因猪 |
| 收稿时间： | 2/8/1996 12:00:00 AM |
| 修稿时间： | 1996/3/27 0:00:00 |
Identification of Integration of Foreign Gene in Transgenic Pigs |
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| Liu Guisheng,Chen Qingxuan,Wang Jing,Wei Qingxin and Chen Yongfu.Identification of Integration of Foreign Gene in Transgenic Pigs[J].Progress In Biochemistry and Biophysics,1996,23(3):279-280. |
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| Authors: | Liu Guisheng Chen Qingxuan Wang Jing Wei Qingxin and Chen Yongfu |
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| Institution: | Institute of Developmental Biology, Academia Sinica;Institute of Developmental Biology, Academia Sinica;Institute of Developmental Biology, Academia Sinica;Institute ofAnimal Husbandry, Hubei Academy of Agricultural Sciences;Institute of Biotechnol, Agricultural University of China |
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| Abstract: | Foreign porcine growth hormone genes were introduced into Hubei white pigs. Inorder to avoid possible homology in metallothionein-Ia gene promoter between sheep and porcine, two PCR primers were designed and located on the regions of sheep metallothionein Ia gene promoter and porcine growth hormone gene, respectively. This reflects the exact results by PCR detection. The results of DNA blotting indicated that 6 of 43 offsprings showed positive integration. |
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| Keywords: | porcine growth hormone gene PCR DNA blotting transgenic pig |
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