黄芪总黄酮对DNA损伤防护作用的研究

用DNA解旋荧光检测法(FADU)研究了黄芪总黄酮(TFA)对γ射线和H₂O₂所致V79细胞DNA链断裂的防护作用. 结果表明TFA对这两种损伤因子所致的DNA损伤均有不同程度的防护作用, 当TFA浓度达到0.4g/L和0.6g/L时, 分别对H₂O₂和γ射线所致的损伤有保护作用(P<0.05), 而浓度增至0.8g/L和1.2g/L时, 分别对两种因素所致的DNA链断裂损伤有非常显著的防护效果(P<0.01), 对H₂O₂的防护效果优于对γ射线.

Protective Effect of Total Flavonoids Astragalus on DNA Strand Breaks in V79 Cells Induced by Hydrogen Peroxide and Gamma Rays

The protective effect of total flavonoids Astragalus (TFA), one of the natural anti-oxide agent isolated from Astragalus monghlious, on DNA strand breaks induced by the hydrogen peroxide and gamma rays has been investigated with the fluorometry assay of DNA unwinding (FADU). The results showed that the TFA has different degree of protective effect on DNA strand breaks caused by hydrogen peroxide and gamma rays respectively. It also showed some protective effect on DNA strand breaks induced by these two damage factors,when the concentration of TFA were 0.4 g/L and 0.6 g/L, respectively. When its concentra-lions were increased to 0.8 g/ L and 1.2 g/L, TFA could effectively protect DNA from hydrogen peroxide and gamma rays damages. Compared the protective effect of TFA on DNA strand breaks induced by hydrogen peroxide and gamma rays, the former was better. The protec-live mechanism of TFA on DNA strand break could be related to savenging free radicals.