| 胞内抗体技术及医学应用 |
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| 引用本文： | 周春水,甄永苏.胞内抗体技术及医学应用[J].生物化学与生物物理进展,1996,23(5):386-390. |
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| 作者姓名： | 周春水 甄永苏 |
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| 作者单位： | 中国医学科学院, 中国协和医科大学医药生物技术研究所;中国医学科学院, 中国协和医科大学医药生物技术研究所 |
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| 摘 要： | 胞内抗体技术是近年来随抗体工程技术发展起来的一项新型基因治疗方法．它将单链抗体表达于非淋巴细胞内，并使之定向分布于细胞核、细胞浆或内质网等部位，特异性阻断、干扰分布于该部位的大分子物质生物活性或加工分泌过程．实验证明该技术能抑制生长因子受体的表达，灭活原癌基因蛋白，抑制病毒复制．在生物科学及医学研究中具有很大应用潜力．
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| 关 键 词： | 抗体工程 胞内抗体 单链抗体 基因治疗 |
| 收稿时间： | 1996/2/14 0:00:00 |
| 修稿时间： | 1996/5/29 0:00:00 |
Intracellular Antibody Technique and Its Medical Application |
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| Zhou Chunshui and Zhen Yongsu.Intracellular Antibody Technique and Its Medical Application[J].Progress In Biochemistry and Biophysics,1996,23(5):386-390. |
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| Authors: | Zhou Chunshui and Zhen Yongsu |
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| Institution: | Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College;Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College |
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| Abstract: | Intracellular antibody refers to the recombinant antibody expressed intracellularly.Single-chain Fv fragment,one common form of intracellular antibody with high affinity,can be expressed in transfected nonlymphocytes and targets to a particular cellular compartment to interfere the activity of some macromolecular substances or the process of their secretion.Intracellular antibody has been proved to be capable of inhibiting growth factor receptors,inactivating oncoproteins and inhibiting HIV-1 replication. Intracellular antibody technique is a novel gene therapy approach with potentiality in medical application. |
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| Keywords: | antibody engineering intracellular targeting single-chain Fv fragment gene therapy |
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