| Sec-10 影响神经肌肉接头突触后敏感性的研究 |
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| 引用本文： | 章雷,李江丽,高尚邦,吴政星,张蓉颖,徐涛.Sec-10 影响神经肌肉接头突触后敏感性的研究[J].生物化学与生物物理进展,2009,36(4):410-416. |
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| 作者姓名： | 章雷 李江丽 高尚邦 吴政星 张蓉颖 徐涛 |
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| 作者单位： | 华中科技大学分子生物物理教育部重点实验室，武汉 430074;华中科技大学分子生物物理教育部重点实验室，武汉 430074;华中科技大学分子生物物理教育部重点实验室，武汉 430074;华中科技大学分子生物物理教育部重点实验室，武汉 430074;华中科技大学分子生物物理教育部重点实验室，武汉 430074;华中科技大学分子生物物理教育部重点实验室，武汉 430074;中国科学院生物物理研究所，生物大分子国家重点实验室，北京 100101 |
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| 基金项目： | 国家自然科学基金资助项目(30500117, 30770523). |
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| 摘 要： | exocyst复合物在分泌过程中起重要作用．可是分子机理尚未研究透彻．通过TMP/UV的基因敲除方法，得到了exocyst复合物中一种组分sec-10的缺陷型线虫．药学结果显示这种线虫神经信号传递存在缺陷，可是电生理的方法证明在神经肌肉接头处的已报道离子型受体和野生型相比并未发生改变．因此猜测，sec-10并未直接影响神经肌肉接头处的离子型受体，而是通过其他途径来行使功能．
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| 关 键 词： | exocyst复合物，sec-10，神经肌肉接头，离子型受体 |
| 收稿时间： | 2009/2/23 0:00:00 |
| 修稿时间： | 2009/3/26 0:00:00 |
Sec-10 Knockout Increases The Neuroactive-drug Responses Without Affecting Function of The Postsynaptic Ionotropic Receptors in Neuromuscular Junctions |
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| ZHANG Lei,LI Jiang-Li,GAO Shang-Bang,WU Zheng-Xing,ZHANG Rong-Ying and XU Tao.Sec-10 Knockout Increases The Neuroactive-drug Responses Without Affecting Function of The Postsynaptic Ionotropic Receptors in Neuromuscular Junctions[J].Progress In Biochemistry and Biophysics,2009,36(4):410-416. |
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| Authors: | ZHANG Lei LI Jiang-Li GAO Shang-Bang WU Zheng-Xing ZHANG Rong-Ying and XU Tao |
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| Institution: | Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China;Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China;Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China;Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China;Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China;Key Laboratory of Molecular Biophysics, Ministry of Education, Huazhong University of Science and Technology, Wuhan 430074, China;National Key Laboratory of Biomacromolecules, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Exocyst complex is known to function in the exocytosis network, however, the molecular mechanism is unclear yet. Using UV/trimethylpsoralen mutagenesis, the sec-10 (one component of the exocyst complex) knockout mutant of C. elegans was obtained for the first time. The drug sensitive assays revealed clearly that the sec-10 gene affected the neural signal transmission, however, the electrophysiological assay showed the function of the ionotropic receptors in the neuromuscular junctions (NMJs) were unaltered compared with the wild type (WT). Thus it was assumed that the sec-10 gene might not influence the known ionotropic receptors in the NMJs, but some other pathways instead. |
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| Keywords: | exocyst complex sec-10 NMJs ionotropic receptors |
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