| 分子诊断技术在乳腺癌检测中的最新进展 |
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| 引用本文： | 邢德纯,王霞,孙锁柱,董莲华,杨靖亚.分子诊断技术在乳腺癌检测中的最新进展[J].生物化学与生物物理进展,2020,47(3):224-232. |
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| 作者姓名： | 邢德纯 王霞 孙锁柱 董莲华 杨靖亚 |
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| 作者单位： | 1)上海海洋大学食品学院,上海 201306;2)中国计量科学研究院前沿计量科学中心,北京 100029,2)中国计量科学研究院前沿计量科学中心,北京 100029,3)火箭军特色医学中心,北京 100088,2)中国计量科学研究院前沿计量科学中心,北京 100029,1)上海海洋大学食品学院,上海 201306 |
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| 基金项目： | 国家重点基础研究发展计划资助项目(2017YFF0204605). |
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| 摘 要： | 乳腺癌是一种严重危害女性健康的恶性肿瘤,对其致病基因的检测有助于肿瘤早期诊断、精准治疗及预后评估.本文总结了近年来乳腺癌相关的热点基因,并对相关基因的分子诊断技术、检测方法及应用进行了综述.首次评述了数字PCR方法用于乳腺癌分子检测的进展.全面对比不同分子诊断技术的差别与优缺点,为乳腺癌关键基因的检测提供指导建议与理论支持,并对未来发展趋势做出展望.
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| 关 键 词： | 乳腺癌基因 生物芯片 核酸分子杂交 数字PCR 高通量测序 |
| 收稿时间： | 2019/10/22 0:00:00 |
| 修稿时间： | 2019/12/24 0:00:00 |
Recent Advances in Molecular Diagnostic Techniques for Breast Cancer Detection |
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| XING De-Chun,WANG Xi,SUN Suo-Zhu,DONG Lian-Hua and YANG Jing-Ya.Recent Advances in Molecular Diagnostic Techniques for Breast Cancer Detection[J].Progress In Biochemistry and Biophysics,2020,47(3):224-232. |
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| Authors: | XING De-Chun WANG Xi SUN Suo-Zhu DONG Lian-Hua and YANG Jing-Ya |
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| Institution: | (College of Food Sciences&Technology,Shanghai Ocean University,Shanghai 201306,China;National Institute of Metrology,Beijing 100029,China;PLA Rocket Force Characteristic Medical Center,Beijing 100088,China) |
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| Abstract: | Breast cancer is a malignant tumor which can seriously endanger women"s health. The detection of its pathogenic genes is helpful for the early detection, accurate treatment and prognosis evaluation of the tumor. This paper summarizes the hot biomarkers related to breast cancer in recent years and reviews the molecular diagnostic techniques, detection methods and applications of related genes. The development of digital PCR in molecular detection of breast cancer was reviewed for the first time. The advantages and disadvantages of different molecular diagnostic techniques were comprehensively compared in this paper. It provides a guidance for the detection of oncogenes in breast cancer, and makes a forecast for future trends. |
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| Keywords: | breast cancer biological chip nucleic acid hybridization digital PCR NGS |
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