| 大鼠卵母细胞体外发育过程中的微管组装研究(英) |
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| 引用本文： | 范衡宇,佟超,李世文,陈大元,孙青原.大鼠卵母细胞体外发育过程中的微管组装研究(英)[J].生物化学与生物物理进展,2002,29(5):708-713. |
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| 作者姓名： | 范衡宇 佟超 李世文 陈大元 孙青原 |
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| 作者单位： | 中国科学院动物研究所，计划生育生殖生物学国家重点实验室，北京 100080;中国科学院动物研究所，计划生育生殖生物学国家重点实验室，北京 100080;中国科学院动物研究所，计划生育生殖生物学国家重点实验室，北京 100080;中国科学院动物研究所，计划生育生殖生物学国家重点实验室，北京 100080;中国科学院动物研究所，计划生育生殖生物学国家重点实验室，北京 100080 |
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| 基金项目： | 国家重点基础研究发展规划项目(973)（G1999055902）和中国科学院知识创新工程重要方向性项目（KSCX2-SW-303）资助. |
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| 摘 要： | 大鼠卵母细胞的发育具有许多与其他哺乳动物不同的特点，用激光共聚焦显微术研究了大鼠卵母细胞发育过程中微管的组装过程，以及一些因素对微管组装过程的影响.结果表明，在大鼠卵母细胞减数分裂的细胞周期进程中，细胞微管系统发生广泛而剧烈的重组，紫杉醇、星形孢菌素和冈田酸等药物能显著改变卵母细胞内的微管组织状态.大鼠卵母细胞可以在体外发生自发的孤雌活化，也可以被细胞松弛素诱导发生胞质假分裂.因此，研究了在这些过程中微管结构的特殊构象，以期更加深入认识这些特殊细胞事件的生化机理.
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| 关 键 词： | 大鼠，卵母细胞，微管，减数分裂 |
| 收稿时间： | 2002/3/11 0:00:00 |
| 修稿时间： | 2002/4/28 0:00:00 |
Microtubule Organization During in Vitro Development of Rat Oocytes |
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| FAN Heng-Yu,TONG Chao,LI Shi-Wen,CHEN Da-Yuan and SUN Qing-Yuan.Microtubule Organization During in Vitro Development of Rat Oocytes[J].Progress In Biochemistry and Biophysics,2002,29(5):708-713. |
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| Authors: | FAN Heng-Yu TONG Chao LI Shi-Wen CHEN Da-Yuan and SUN Qing-Yuan |
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| Institution: | State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China;State Key Laboratory of Reproductive Biology, Institute of Zoology, The Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | Microtubule organization in developing rat oocytes were investigated through confocal microscopy. The inductive effects of some factors on microtubule modification were also studied. The results indicated that the meiotic cell cycle progression of rat oocyte is accompanied by extensive rearrangement of the microtubule network of the cell. Microtubule organization was modified by the treatment of oocytes with taxol, staurosporine, or okadaic acid. Rat oocytes released from the oviducts could undergo spontaneous parthenogenetic activation. They could also be induced to pseudo-cleave by cytochalasin B treatment. The microtubule configuration in rat oocytes undergoing spontaneous parthenogenetic activation or pseudo-cleavage was also observed. |
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| Keywords: | rat oocyte microtubule meiosis |
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