体外分子定向进化研究进展

体外定向进化作为近几年发展起来的一种蛋白质改造新策略，可以在未知目标蛋白三维结构信息和作用机制的情况下，通过对编码基因的随机突变、重组和定向筛选，获得具有改进功能或全新功能的蛋白质，使几百万年的自然进化过程在短期内得以实现，因而是发现新的生物活性分子和反应途径的重要方法，已在短短几年内取得了令人瞩目的成就.

Recent Advances in In vitro Molecular Directed Evolution

In vitro molecular directed evolution, based on the combination of random mutagenesis, recombination and high throughput screening, is a new strategy of improving protein properties. With this technology, the properties of the target proteins, such as specificity, catalytic activity and affinity can be tuned without the knowledge of 3-D structure information and function mechanisms. Recently, the methodology originating from error prone PCR and DNA shuffling has been greatly developed by many new techniques. Here, based on the literature review and the experience of authors, the recent developments have been reviewed.