| 碱性磷酸酶标记链霉亲和素 |
| |
| 引用本文： | 赵启仁,李美佳,刘洁,宋娜玲,庄湘莲,陈艾.碱性磷酸酶标记链霉亲和素[J].生物化学与生物物理进展,1999,26(4):391-393. |
| |
| 作者姓名： | 赵启仁 李美佳 刘洁 宋娜玲 庄湘莲 陈艾 |
| |
| 作者单位： | 1. 中国医学科学院,放射医学研究所,天津,300192
2. 中国协和医科大学,放射医学研究所,天津,300192 |
| |
| 基金项目： | 国家自然科学基金资助课题(39770216). |
| |
| 摘 要： | 碱性磷酸酶标记链霉亲和素（AP-SA）是酶放大时间分辨荧光免疫分析（EATRFIA）通用的、最关键的试剂.报告了AP-SA的戊二醛二步标记方法.用自行研制的荧光发展溶液和AP的底物5-氟水杨酸磷酸酯测定了标记物AP-SA的特性，AP的标记回收率为38.7%；AP-SA稀释200～12 800倍时，稀释倍数和Tb的信/噪比呈线性关系；至少在两个月内，AP-SA的酶活性是稳定的.
|
| 关 键 词： | 酶放大时间分辨荧光免疫分析 碱性磷酸酶 链霉亲和素 |
| 收稿时间： | 5/5/1998 12:00:00 AM |
| 修稿时间： | : 1998-05- |
Study of Alkaline Phosphatase-labelled Streptavidin |
| |
| ZHAO Qi-Ren,LI Mei-Ji,LIU Jie,SONG Na-Ling,ZHUANG Xiang-Lian and CHEN Ai.Study of Alkaline Phosphatase-labelled Streptavidin[J].Progress In Biochemistry and Biophysics,1999,26(4):391-393. |
| |
| Authors: | ZHAO Qi-Ren LI Mei-Ji LIU Jie SONG Na-Ling ZHUANG Xiang-Lian and CHEN Ai |
| |
| Institution: | Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China;Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China;Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China;Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China;Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China;Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China |
| |
| Abstract: | |
| |
| Keywords: | enzyme-amplified time-resolved fluoroimmunoassay alkaline phosphatase streptavidin |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
