| 用低浓度硫氰酸胍提取高质量植物RNA |
| |
| 引用本文： | 何军贤,梁厚果.用低浓度硫氰酸胍提取高质量植物RNA[J].生物化学与生物物理进展,1998,25(4):379-381. |
| |
| 作者姓名： | 何军贤 梁厚果 |
| |
| 作者单位： | 四川大学生物系, 成都 610064;四川大学生物系, 成都 610064 |
| |
| 基金项目： | 国家自然科学基金资助项目(39770446). |
| |
| 摘 要： | 目前分离RNA的方法很多, 但大多是基于动物材料建立的方法, 而针对植物材料的方法并不多见. 建立了一种从植物材料中分离高质量RNA的硫氰酸胍/氯化锂/热酚法. 与其他硫氰酸胍的方法相比, 该方法所用硫氰酸胍的浓度仅为现有方法的1/40 (0.1 mol/L),降低了实验成本, 且所得RNA质量令人满意. 用该方法分离的RNA在琼脂糖凝胶电泳上可清晰分出4条核糖体RNA (rRNA)带; 用此RNA进行RNA印迹或分离mRNA进行体外翻译试验, 均获得很好效果.
|
| 关 键 词： | RNA分离，硫氰酸胍，氯化锂，植物 |
| 收稿时间： | 1997/6/23 0:00:00 |
| 修稿时间： | 1998/1/19 0:00:00 |
Preparation of High Quality Plant RNA With Low Concentration of Guanidinium Thiocyanate |
| |
| HE Jun-xian and LIANG Hou-guo.Preparation of High Quality Plant RNA With Low Concentration of Guanidinium Thiocyanate[J].Progress In Biochemistry and Biophysics,1998,25(4):379-381. |
| |
| Authors: | HE Jun-xian and LIANG Hou-guo |
| |
| Institution: | Department of Biology, Sichuan University, Chengdu 610064, China;Department of Biology, Sichuan University, Chengdu 610064, China |
| |
| Abstract: | The guanidinium thiocyanate-LiCl-hot phenol method for high purity and high integrity RNA isolation from plant tissues was established. Comparing with other methods that involved guanidinium thiocyanate, this method costs less and produces RNA molecules with better quality. The working concentration of guanidinium thiocyanate used was reduced more than 40 times compared with previous methods. The isolated RNA with this method gave 4 ribosomal RNA (rRNA) bands when analysed by formaldehyde agarose gel electrophoresis. Northern hybridization, mRNA isolation and following in vitro translation experiments performed with this RNA also gave good results. |
| |
| Keywords: | RNA isolation guanidinium thiocyanate LiCl plant tissues |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
