| 人神经营养因子4基因的分子克隆及序列分析 |
| |
| 引用本文： | 王爱坤,刘哲伟,包怡华.人神经营养因子4基因的分子克隆及序列分析[J].生物化学与生物物理进展,1998,25(2):183-185. |
| |
| 作者姓名： | 王爱坤 刘哲伟 包怡华 |
| |
| 作者单位： | 首都儿科研究所, 北京市高技术实验室,北京 100020;首都儿科研究所, 北京市高技术实验室,北京 100020;首都儿科研究所, 北京市高技术实验室,北京 100020 |
| |
| 摘 要： | 以正常人血淋巴细胞染色体DNA为模板,PCR扩增出神经营养因子4(NT4)编码基因.将所得基因片段重组于噬菌体载体M13mp18RF,筛选得到含人NT4基因的克隆.采用Sanger单链末端终止法测出其全部的核苷酸序列,该序列与国外文献所报道的完全一致.
|
| 关 键 词： | 神经营养因子4 基因克隆 序列分析 |
| 收稿时间： | 1997/3/12 0:00:00 |
| 修稿时间： | 1997/7/17 0:00:00 |
Molecular Cloning and Sequencing of Neurotrophin-4 Gene from Chinese |
| |
| WANG Ai-kun,LIU Zhe-wei and BAO Yi-hua.Molecular Cloning and Sequencing of Neurotrophin-4 Gene from Chinese[J].Progress In Biochemistry and Biophysics,1998,25(2):183-185. |
| |
| Authors: | WANG Ai-kun LIU Zhe-wei and BAO Yi-hua |
| |
| Institution: | Beijing Municipal High-tech Laboratory,Capital Institute of Pediatrics, Beijing 100020, China;Beijing Municipal High-tech Laboratory,Capital Institute of Pediatrics, Beijing 100020, China;Beijing Municipal High-tech Laboratory,Capital Institute of Pediatrics, Beijing 100020, China |
| |
| Abstract: | With the chromosomal DNA of human blood lymphocytes as template, neurotrophin-4 coding genes were amplified by PCR and recombinated into phage vector M13, which were sequenced by using Sanger's single-stranded DNA terminal termination method. The sequence of the cloned gene is completely the same as that reported in the literature. |
| |
| Keywords: | neurotrophin-4 gene cloning DNA sequencing |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
