| GPx对活性氧诱发细胞程序性死亡的影响 |
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| 引用本文： | 孙娟,陈瑗,周玫.GPx对活性氧诱发细胞程序性死亡的影响[J].生物化学与生物物理进展,1998,25(3):259-262. |
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| 作者姓名： | 孙娟 陈瑗 周玫 |
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| 作者单位： | 第一军医大学自由基医学研究室,广州 510515;第一军医大学自由基医学研究室,广州 510515;第一军医大学自由基医学研究室,广州 510515 |
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| 摘 要： | 谷胱甘肽过氧化物酶（GPx）是细胞内清除活性氧的主要抗氧化酶之一.以稳定表达GPx的CHO细胞系为模型，研究GPx对百草枯（paraquat）和叔丁基脂氢过氧化物（tbOOH）细胞毒性的影响，发现paraquat和tbOOH都能够诱导CHO细胞产生典型的细胞程序性死亡的形态学改变和特征性的DNA“梯子状”断裂，而稳定表达GPx的细胞系能明显抵抗tbOOH诱发的细胞程序性死亡，但不能抵抗paraquat诱发的细胞程序性死亡.该结果揭示，GPx能选择性抑制活性氧诱发的细胞凋亡.
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| 关 键 词： | 谷胱甘肽过氧化物酶 基因转染 百草枯 叔丁基脂氢过氧化物 细胞程序性死亡 |
| 收稿时间： | 1997/3/13 0:00:00 |
| 修稿时间： | 1997/6/10 0:00:00 |
Effect of Glutathione Peroxidase on Programmed Cell Death Induced by Reactive Oxygen Species |
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| SUN Juan,CHEN Yuan and ZHOU Mei.Effect of Glutathione Peroxidase on Programmed Cell Death Induced by Reactive Oxygen Species[J].Progress In Biochemistry and Biophysics,1998,25(3):259-262. |
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| Authors: | SUN Juan CHEN Yuan and ZHOU Mei |
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| Institution: | Laboratory of Free Radical Medicine, The First Military Medical University, Guangzhou 510515, China;Laboratory of Free Radical Medicine, The First Military Medical University, Guangzhou 510515, China;Laboratory of Free Radical Medicine, The First Military Medical University, Guangzhou 510515, China |
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| Abstract: | Glutathione peroxidase (GPx) is one of the major cellular antioxidant enzymes in scavenging reactive oxygen species (ROS). The effects of GPx on cytotoxicity mediated by paraquat and t-butylhydroperoxide (tbOOH) in GPx overexpressing in cell lines were investigated. It was found that both paraquat and tbOOH could induce the typical features of programmed cell death (PCD) including chromatin condensation and DNA fragmentation. The GPx expressing cell clones could effciently inhibit PCD induced by tbOOH, but not by paraquat. The results suggested that GPx selectively inhibits cytotoxicity induced by ROS. |
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| Keywords: | glutathione peroxidase gene transfection paraquat t-butylhydroperoxide (tbOOH) programmed cell death |
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