| 一种丙烯酰胺凝胶的新型光聚合法 |
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| 引用本文： | 周军贤,周筠梅.一种丙烯酰胺凝胶的新型光聚合法[J].生物化学与生物物理进展,1998,25(2):167-170. |
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| 作者姓名： | 周军贤 周筠梅 |
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| 作者单位： | 中国科学院生物物理研究所，生物大分子国家重点实验室，北京 100101;中国科学院生物物理研究所，生物大分子国家重点实验室，北京 100101 |
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| 摘 要： | 以亚甲基蓝为引发剂，甲苯亚磺酸钠为还原剂，二苯氯化碘为氧化剂的丙烯酰胺凝胶光聚合方法，具有试剂配制简单, 适用范围广，方法稳定性和重现性好，且聚合速度快，操作易于控制等特点, 是一种不同于最为广泛使用的过硫酸铵/四甲基乙二胺和核黄素/四甲基乙二胺法的新型光聚合法. 用这一方法做了SDS和在酸性缓冲体系中的尿素变性梯度胶电泳，初步试用表明这一新型光聚合法具有很好的聚合效果.
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| 关 键 词： | 亚甲基蓝，光聚合，聚丙烯酰胺凝胶电泳 |
| 收稿时间： | 1996/12/28 0:00:00 |
| 修稿时间： | 1997/4/28 0:00:00 |
A New Photopolymerization System for Polyacrylamide Gel |
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| ZHOU Jun-xian and ZHOU Jun-mei.A New Photopolymerization System for Polyacrylamide Gel[J].Progress In Biochemistry and Biophysics,1998,25(2):167-170. |
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| Authors: | ZHOU Jun-xian and ZHOU Jun-mei |
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| Institution: | National Laboratory of Biomacromolecules Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China;National Laboratory of Biomacromolecules Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | A method for photopolymerization of polyacrylamide gel with methlene blue (initiator), sodium toluenesulfinate (reducer) and diphenyl-iodonium chloride (oxidizer) features simple operation, reliable gelation, good reproducibility and easy control of gelation, which is advocated as a valid alteranative to the most popularly used AP/TEMED and Rf/TEMED ones. This method was tested for SDS and uDGGE with acidic buffer system, and satisfactory results were obtained. These preliminary experiments demonstrate that this novel method of photopolymerization gives reliable results and is another choice for gel polymerization. |
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| Keywords: | methylene blue photopolymerization polyacrylamide gel electrophoresis |
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