| 固定化尿酸酶丝素膜的性质及其尿酸传感器 |
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| 引用本文： | 张雨青,顾仁敖,朱江,曹佩根.固定化尿酸酶丝素膜的性质及其尿酸传感器[J].生物化学与生物物理进展,1998,25(3):275-278. |
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| 作者姓名： | 张雨青 顾仁敖 朱江 曹佩根 |
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| 作者单位： | 苏州大学蚕桑学院，苏州 215151;苏州大学化学化工学院，苏州 215151;苏州大学水产学院，苏州 215151;苏州大学化学化工学院，苏州 215151 |
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| 基金项目： | 江苏省教委自然科学基金资助课题(970054). |
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| 摘 要： | 应用电化学分析法对固定化酶丝素膜的性质进行了分析，结果表明这种酶经丝素膜固定后，活性得率高、性能稳定、能长期存放.用这种酶膜和氧电极等组成的流动注射分析式尿酸传感器对生物样品进行的百次重复分析结果表明，这种传感器的重现性良好，每小时能分析60个人血清样品.
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| 关 键 词： | 固定化尿酸酶丝素膜，尿酸传感器，氧电极，流动注射分析 |
| 收稿时间： | 1997/5/13 0:00:00 |
| 修稿时间： | 1997/9/22 0:00:00 |
The Characterization of Uricase-Immobilized Silk Fibroin Membrane and Its Application to Biosensor for Uric Acid |
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| ZHANG Yu-qing,GU Ren-ao,ZHU Jiang and CAO Pei-gen.The Characterization of Uricase-Immobilized Silk Fibroin Membrane and Its Application to Biosensor for Uric Acid[J].Progress In Biochemistry and Biophysics,1998,25(3):275-278. |
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| Authors: | ZHANG Yu-qing GU Ren-ao ZHU Jiang and CAO Pei-gen |
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| Institution: | School of Sericulture, Suzhou University, Suzhou 215151, China;School of Sericulture, Suzhou University, Suzhou 215151, China;School of Sericulture, Suzhou University, Suzhou 215151, China;School of Sericulture, Suzhou University, Suzhou 215151, China |
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| Abstract: | Uricase-immobilized silk fibroin membrane was characterized by means of electrochemistry method. When uricase was immobilized in silk fibroin membrane, the immobilized uricase was improved largely at its stability to heat and pH, and it can be stored for long time. Based on this uricase membrane and oxygen electrode, an amperometric sensor has been developed for measurement of uric acid in flow injection analysis. The biosensor has a good reproducibility for repeatedly monitoring biosamples for 100 times respectively and is capable of detecting over 60 serum samples per hour. |
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| Keywords: | uricase-immobilized fibroin membrane urate sensor oxygen electrode flow injection analysis |
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