电场驱动微悬臂生物传感器及对DNA 的快速、高灵敏度检测

微悬臂列阵传感器在生物检测方面具有快速、痕量和非标记的特性. 我们以镀金并在其上固定了 DNA 探针的微悬臂为正极，在靶杂交液槽内引入另一电极作为负极，构成电场驱动微悬臂 DNA 生物传感器. 对该传感器系统施加静电场，驱动 DNA 分子朝正极迁移，使溶液中的 DNA 分子富集在微悬臂上，促进 DNA 分子的杂交. 结果表明： a. DNA 在微悬臂上的杂交时间仅需 3 min，加快了微悬臂生物传感器对 DNA 分子的检测速度； b. 提高了微悬臂生物传感器的灵敏度，可以检测到皮克级的 DNA 分子.

Electrostatic Microcantilever Array Biosensor and Its Application in DNA Detection

Microcantilever array biosensor shows peculiarities of high speed, trace detection and label-free in bioanalysis. A method to increase the detection sensitivity and to speed up analysis of microcantilever array biosensor is reported. The gold covered microcantilever array immobilized by DNA probes is applied as a microcantilever array biosensor with electrostatic field. Microcantilever arry is acted as anode in hybridization solution cell where DNA molecules are removed to and congregated around anode, so the DNA hybridization is enhanced. The data demonstrated: (1) the time of DNA hybridization on the cantilever system drived by direct electric field was no more than 3 min; (2) the detection sensitivity was improved from 30 ng/L to 300 ng/L level.