肝癌细胞系差异性表达的糖结合蛋白研究

糖结合蛋白(glycan-binding protein,GBP)在细胞生命周期中扮演着重要角色,如细胞识别、运输、免疫、代谢、增殖分化及细胞间的相互作用等.目前,对GBP的改变对细胞生物过程产生影响的研究甚少.本研究用糖芯片技术对肝癌细胞系Hep G2和正常肝细胞系L02表达的GBP进行研究;糖细胞化学验证确定差异表达GBP在肝癌细胞系中的变化和分布.结果显示,8种糖探针(如SL、LNT和Gal NAc等)和5种糖探针(如Man、Man-9-Glycan,Xyl等)分别对应的GBP在Hep G2细胞中表达上调或下调.糖细胞化学结果显示:Gal NAc识别的GBPs主要表达在Hep G2的胞膜、中央胞质、核周胞质区域,而在L02的相同区域表达减弱;Neu Ac识别的GBPs主要表达在L02的胞膜区及核周胞质区,而在Hep G2细胞的相同区域表达减弱.这些数据为寻找新的肝癌发病机制和抗肿瘤策略提供了有用信息.

Altered Expression of Glycan-binding Protein in Hepatocellular Carcinoma Cell Lines

Glycan-binding protein play important biological roles in biological processes. We use carbohydrate microarray to study the alteration of GBP in hepatocellular carcinoma cell line HepG2 and L02. Carbohydrate histochemistry was used to further validate the GBP and assess the distribution. As a result, 8 carbohydrate probes (e. g. SL, LNT, and GalNAc) showed increased signal while 5 carbohydrate probes (e. g. Man, Man-9-Glycan, and Xyl) showed decreased signal in HepG2 compared with L02 cell line. Meanwhile, GalNAc staining showed moderate binding to the cytoplasma membrane, central cytoplasm, and perinuclear cytoplasm in the L02, and the binding intensified in the same regions of the HepG2. NeuAc staining showed moderate binding to the cytoplasma membrane, and perinuclear cytoplasm in the HepG2, and the binding intensified in the same regions of the L02. In conclusion, the precision alteration of GBP related to HepG2 may provide useful information to find new molecular mechanism of hepatocellular carcinoma and antitumor therapeutic strategies.