| 胃肠富集Kruppel样因子在食管癌的表达 |
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| 引用本文： | 王楠,刘芝华,丁芳,王秀琴,周传农,王明荣,吴旻.胃肠富集Kruppel样因子在食管癌的表达[J].生物化学与生物物理进展,2002,29(1):46-50. |
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| 作者姓名： | 王楠 刘芝华 丁芳 王秀琴 周传农 王明荣 吴旻 |
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| 作者单位： | 中国医学科学院肿瘤研究所，分子肿瘤学国家重点实验室，北京，100021 |
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| 基金项目： | 国家自然科学基金资助项目(39993420)和国家重点基础研究发展规划资助项目(G1998051021). |
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| 摘 要： | 胃肠富集Kruppel样因子（GKLF）是一个新近发现的真核锌指蛋白，它在胃肠道表达丰富，其表达与细胞生长停滞有关联.用半定量的RT-PCR的方法，比较了食管鳞癌病人癌组织和正常粘膜的GKLF表达.17例食管鳞癌病人均检测到GKLF mRNA的表达，其中14例癌组织中GKLF表达比临近正常组织减少.人原代培养成纤维细胞中GKLF的去血清诱导作用明显，而在一食管鳞癌细胞系EC9706中该诱导作用减弱.对EC9706细胞GKLF cDNA的序列分析表明，该基因的cDNA编码区未发生突变.结果证实，食管鳞癌中GKLF表达下调.
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| 关 键 词： | 食管癌，胃肠富集Kruppel样因子，RT-PCR，基因表达 |
| 收稿时间： | 2001/9/25 0:00:00 |
| 修稿时间： | 2001年9月25日 |
Expression of Gut-enriched Kruppel-like Factor in Esophageal Squamous Cancer |
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| WANG Nan,LIU Zhi-Hu,DING Fang,WANG Xiu-Qin,ZHOU Chuan-Nong,WANG Ming-Rong and WU Min.Expression of Gut-enriched Kruppel-like Factor in Esophageal Squamous Cancer[J].Progress In Biochemistry and Biophysics,2002,29(1):46-50. |
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| Authors: | WANG Nan LIU Zhi-Hu DING Fang WANG Xiu-Qin ZHOU Chuan-Nong WANG Ming-Rong and WU Min |
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| Institution: | National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China;National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China;National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China;National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China;National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China;National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China;National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing 100021, China |
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| Abstract: | Gut-enriched Kruppel-like factor (GKLF) is a newly identified eukaryotic zinc finger protein expressed extensively in the gastrointestinal tract, the expression of which is associated with growth arrest. GKLF expression in the tumor was compared to normal mucosa in esophageal squamous cancer patients, using semi-quantitative RT-PCR. Expression of GKLF mRNA was detected all of 17 cases of esophageal squamous cancer. Decreased expression of GKLF mRNA was observed in 14 of esophageal squamous cancer patients compared to normal esophageal tissues adjacent to tumor from the same patient. Serum deprivation induced overexpression of GKLF was observed in the primary culture of human fibroblast and this induction was decreased in an esophageal squamous cancer cell line EC9706. Sequencing of GKLF cDNA from EC9706 showed no mutations in the coding region. The results demonstrate that GKLF expression in esophageal squamous cancer is down-regulated. |
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| Keywords: | esophageal cancer gut-enriched Kruppel-like factor RT-PCR gene expression |
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