六种重金属离子胁迫诱导鱼类细胞凋亡的研究

以草鱼（Ctenopharyngodon idellus）ZC7901细胞为研究模型，选用六种重金属离子进行胁迫诱导鱼类细胞凋亡试验.结果显示，在Cd²⁺、Cr⁶⁺、Hg²⁺、Cu²⁺、As⁵⁺、Pb²⁺的胁迫作用下，ZC7901细胞均出现了明显的染色质凝集、趋边化、形成凋亡小体等凋亡形态特征，核酸电泳显示DNA发生特异降解而呈现电泳阶梯，用末端脱氧核糖核酸转移酶介导的dUTP切口末端标记（TUNEL）法，检测到DNA的3′-OH断端均被原位特异标记，表明六种重金属离子均能诱导鱼类细胞发生典型的凋亡.

Apoptosis Induction in Fish Cells under Stress of Six Heavy Metal Ions

A grass carp ( Ctenopharyngodon idellus )cell culture ZC7901 was used as an in vitro model system to study the effect of heavy metal ions on fish cells. The results showed that the cell culture chemically stressed by six heavy metal ions, Cd 2 ,Cr 6 ,Hg 2 ,Cu 2 ,As 5 and Pb 2 , exhibited cell death accompanied by various morphological alterations such as nuclear condensation, chromatin abutted sharply against nuclear membrane and developed into distinct apoptotic like bodies, which were characteristic features seen in apoptosis. By agarose gel electrophoresis, DNA ladder were clearly visualized, which indicated the specific degradation of DNA. Also terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) analysis of the cells demonstrated DNA fragmentation localized in the dying cells as well as in the additional apoptotic like bodies. All the results indicated that six heavy metal ions, Cd 2 ,Cr 6 ,Hg 2 ,Cu 2 ,As 5 and Pb 2 , were all able to induce apoptosis in fish cells.