| 肺腺癌靶向性超氧化物歧化酶的构建及功能分析 |
| |
| 引用本文： | 卢 敏,龚兴国,汪辰卉,郑 乐,郭建军,章申峰.肺腺癌靶向性超氧化物歧化酶的构建及功能分析[J].生物化学与生物物理进展,2006,33(1):51-58. |
| |
| 作者姓名： | 卢 敏 龚兴国 汪辰卉 郑 乐 郭建军 章申峰 |
| |
| 作者单位： | 浙江大学生命科学学院生物大分子与酶工程研究所,杭州,310027 |
| |
| 摘 要： | 临床上,超氧化物歧化酶(SOD)的低肿瘤细胞定位能力限制了其在抗肿瘤领域的广泛应用,这一直是国内外学者们试图解决的难点.研究中,以基因工程方法连接念珠藻Fe-SOD(iron-superoxidedismutase)基因和抗SPC-A-1肺腺癌LC-1ScFv(singlechainFv)基因,并融合表达获得了SOD-ScFv融合蛋白.纯化后SOD-ScFv表现出SOD和ScFv的双重活性.SPC-A-1肺腺癌细胞中,融合蛋白的异硫氰酸荧光素(FITC)染色追踪和自由基含量分析表明,SOD-ScFv具备识别SPC-A-1肺腺癌细胞、透膜并清除胞内自由基的功能,最终达到抑制肿瘤细胞生长的目的.研究提出的靶向抗肿瘤机制将克服临床上SOD无目标趋向性和难于进入实体瘤的两大应用局限性,并提供了一种利用LC-1ScFv来靶向投递抗肿瘤药物的思路.
|
| 关 键 词： | Fe-SOD 单链抗体(ScFv) 融合表达 靶向治疗 |
| 收稿时间： | 2005-06-13 |
| 修稿时间： | 2005-06-132005-07-29 |
Construction and Functional Characterization of Lung Adenocarcinoma Targeting SOD |
| |
| LU Min,GONG Xing-Guo,WANG Chen-Hui,ZHENG Le,GUO Jian-Jun and ZHANG Shen-Feng.Construction and Functional Characterization of Lung Adenocarcinoma Targeting SOD[J].Progress In Biochemistry and Biophysics,2006,33(1):51-58. |
| |
| Authors: | LU Min GONG Xing-Guo WANG Chen-Hui ZHENG Le GUO Jian-Jun and ZHANG Shen-Feng |
| |
| Institution: | Institute of Biologic Macromolecule and Enzyme Engineering, College of Life Science, Zhejiang University, Hangzhou 310027, China;Institute of Biologic Macromolecule and Enzyme Engineering, College of Life Science, Zhejiang University, Hangzhou 310027, China;Institute of Biologic Macromolecule and Enzyme Engineering, College of Life Science, Zhejiang University, Hangzhou 310027, China;Institute of Biologic Macromolecule and Enzyme Engineering, College of Life Science, Zhejiang University, Hangzhou 310027, China;Institute of Biologic Macromolecule and Enzyme Engineering, College of Life Science, Zhejiang University, Hangzhou 310027, China;Institute of Biologic Macromolecule and Enzyme Engineering, College of Life Science, Zhejiang University, Hangzhou 310027, China |
| |
| Abstract: | In clinic, the low tumour-targeted ability of SOD is a critical shortage in its application, which is adifficulty for scientist at all time. For solving this problem, Nostoc commune CHEN iron-superoxide dismutase(Fe-SOD) and anti-lung adenocarcinoma LC-1 single chain Fv (ScFv) were fused, and the fusion protein namedSOD-ScFv was expressed. After purification, fusion protein demonstrated both SOD and LC-1 antibody activities.The result of tracing of SOD-ScFv by FITC dyeing and quantification of ROS(reactive oxygan species) in SPC-A-1(lung adenocarcinoma) cells showed that the fusion protein possessed the ability to recognize SPC-A-1 cells andeliminate the cellular ROS. The tumour-targeted theory put up in this research will overcome two applieddisadvantages of SOD in clinic: it neither target the tumour cell nor permeates through the cell membrane. Also,the research provides a feasible idea that ScFv can be used to target the anticancer drug to tumour. |
| |
| Keywords: | Fe-SOD single chain antibody (ScFv) fusion expression target therapy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
