| 微流控芯片技术在循环肿瘤细胞分离中的研究进展 |
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| 引用本文： | 吕晓庆,李雷,陈红梅,陈鹏,刘静.微流控芯片技术在循环肿瘤细胞分离中的研究进展[J].生物化学与生物物理进展,2015,42(4):301-312. |
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| 作者姓名： | 吕晓庆 李雷 陈红梅 陈鹏 刘静 |
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| 作者单位： | 天津医科大学基础医学研究中心转化肿瘤学实验室，天津 300070,中国科学院理化技术研究所低温工程学重点实验室，低温生物医学工程学北京市重点实验室，北京 100190,中国科学院半导体研究所集成光电子学国家重点联合实验室，北京 100083,天津医科大学附属肿瘤医院，天津市肺癌诊治中心，天津 300060,中国科学院理化技术研究所低温工程学重点实验室，低温生物医学工程学北京市重点实验室，北京 100190 |
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| 基金项目： | 中国科学院重点部署项目 (KJZD-EW-TZ-L03-6)，天津市自然科学基金项目(13JCYBJC23600)，国家自然科学基金面上项目(61078074)和中国博士后科学基金(2014M550794)资助 |
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| 摘 要： | 循环肿瘤细胞(circulating tumor cells,CTCs)是指从原发肿瘤或转移灶脱落、发生上皮-间质转化进入患者外周血血液循环的恶性肿瘤细胞.CTCs在肿瘤研究和临床诊断上的作用逐渐得到认可,外周血中CTCs存在与否以及数量多少不但可以用于肿瘤的早期诊断,还可以用于评估肿瘤预后、监测肿瘤的转移和复发.微流控芯片作为一个高通量、小型化的细胞实验平台,已被应用于CTCs的分选当中.本文综述了用于CTCs捕获的微流控芯片系统的最新研究进展,着重介绍各类芯片的捕获原理、芯片结构和捕获效率,最后对微流控芯片技术在CTCs分选中的应用前景进行了展望.
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| 关 键 词： | 微流控芯片 循环肿瘤细胞 肿瘤转移 细胞分选 |
| 收稿时间： | 2014/10/22 0:00:00 |
| 修稿时间： | 3/3/2015 12:00:00 AM |
Advances in Isolating Circulating Tumor Cells With Microfluidic Chips |
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| LV Xiao-Qing,LI Lei,CHEN Hong-Mei,CHEN Peng and LIU Jing.Advances in Isolating Circulating Tumor Cells With Microfluidic Chips[J].Progress In Biochemistry and Biophysics,2015,42(4):301-312. |
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| Authors: | LV Xiao-Qing LI Lei CHEN Hong-Mei CHEN Peng and LIU Jing |
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| Institution: | Research Center of Basic Medical Science, Tianjin Medical University, Tianjin 300070, China,Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences & Beijing Key Laboratory of Cryo-Biomedical Engineering, Beijing 100190, China,Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China,Center for Lung Cancer Diagnosis and Treatment, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China and Key Laboratory of Cryogenics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences & Beijing Key Laboratory of Cryo-Biomedical Engineering, Beijing 100190, China |
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| Abstract: | Circulating tumor cells (CTCs) are shed by primary or metastatic tumors, undergo the epithelial-mesenchymal transition, and enter into the peripheral blood of patients with metastatic cancers. Its critical roles in cancer research and clinical diagnostics are gradually being recognized. The presence of CTCs, especially the number in peripheral blood can not only be used for early diagnosis of cancer but also be used to assess prognosis, and monitor tumor metastasis and recurrence. Microfluidic chip as a high-throughput, miniaturized cell experimental platform has been used in CTCs sorting. In this review, we highlight recent progresses of CTCs capturing of microfluidic chip system and focus on the capture theory, chip structure and capture efficiency of various types of chips. At last, we prospect for the application foreground of microfluidic chip technology in CTCs isolation. |
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| Keywords: | microfluidic chip circulating tumor cells (CTCs) tumor metastasis cell sorting |
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