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| 正常与重金属铅注射的兔脑蛋白质双向电泳图谱比较与鉴定 | |
| 引用本文： | 赵锐,纪建国,郝守进,陈倩,袁洪生,管华诗,茹炳根.正常与重金属铅注射的兔脑蛋白质双向电泳图谱比较与鉴定[J].生物化学与生物物理进展,2001,28(6):874-879. |
| 作者姓名： | 赵锐 纪建国 郝守进 陈倩 袁洪生 管华诗 茹炳根 |
| 作者单位： | 1. 北京大学生命科学学院,蛋白质工程及植物基因工程国家重点实验室,北京,100871;青岛海洋大学海洋药物与食品研究所,青岛,266003 2. 北京大学生命科学学院,蛋白质工程及植物基因工程国家重点实验室,北京,100871 3. 青岛海洋大学海洋药物与食品研究所,青岛,266003 |
| 摘 要： | 重金属污染对人类健康的威胁日益受到关注，为了了解大量重金属摄入对脑蛋白质的影响，对比研究了正常兔脑组织蛋白质与重金属铅腹腔注射2周后的兔脑组织在蛋白质双向电泳图谱中的差异，分析重金属注射对脑蛋白质表达的可能影响.通过对脑组织蛋白质的提取，分离出水溶性的蛋白质组分，经双向电泳图谱比较正常与注射重金属铅的兔子在脑蛋白质表达上的差异，其中3个蛋白质斑点经提取，反相高效液相色谱(RP-HPLC)分离，基质辅助激光解析电离质谱(MALDI-TOF MS)确定了分子质量,并利用肽质量指纹图谱检索数据库确定蛋白质的归属.实验结果表明正常兔脑与金属铅注射的兔脑在水溶性蛋白质的表达上具有显著性差异. |
| 关 键 词： | 蛋白质组学，双向电泳，基质辅助激光解析电离质谱，电喷雾质谱 脑蛋白 |
| 收稿时间： | 2001/3/19 0:00:00 |
| 修稿时间： | 2001年3月19日 |
Comparison and Identification of Rabbit Brain Proteins from the Normal and PbCl2 Injected Rabbit by High-resolution Two-dimensional Gel Electrophoresis and Mass Spectrometry |
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| ZHAO Rui,JI Jian-Guo,HAO Shou-Jin,CHEN Qian,YUAN Hong-Sheng,GUAN Hua-Shi and RU Bing-Gen.Comparison and Identification of Rabbit Brain Proteins from the Normal and PbCl2 Injected Rabbit by High-resolution Two-dimensional Gel Electrophoresis and Mass Spectrometry[J].Progress In Biochemistry and Biophysics,2001,28(6):874-879. | |
| Authors: | ZHAO Rui JI Jian-Guo HAO Shou-Jin CHEN Qian YUAN Hong-Sheng GUAN Hua-Shi and RU Bing-Gen |
| Institution: | National Laboratory of Protein Engineering and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, China;Institute of Marine Drugs and Foods, Ocean University of Qingdao, Qingdao 266003, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, China;Institute of Marine Drugs and Foods, Ocean University of Qingdao, Qingdao 266003, China;National Laboratory of Protein Engineering and Plant Genetic Engineering, School of Life Science, Peking University, Beijing 100871, China |
| Abstract: | |
| Keywords: | proteome two-dimension gel electrophoresis matrix-associated laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS) electrospray ionization mass spectrometry(ESI-MS) brain protein |
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