| 从少量转染细胞中同时快速提取总RNA和基因组DNA |
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| 引用本文： | 张宏权,王会信,周廷冲,王允玲.从少量转染细胞中同时快速提取总RNA和基因组DNA[J].生物化学与生物物理进展,1994,21(2):165-166. |
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| 作者姓名： | 张宏权 王会信 周廷冲 王允玲 |
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| 作者单位： | 北京军事医学科学院基础医学研究所;北京军事医学科学院基础医学研究所;北京军事医学科学院基础医学研究所;北京军事医学科学院基础医学研究所 |
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| 基金项目： | 国家自然科学基金（青年）39100065号资助. |
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| 摘 要： | 采用4mol / L LiCl将DNA和RNA分相，建立了同时从少量转染细胞中快速提取细胞总RNA和大分子基因组DNA的方法.与以前的方法相比，本法快速、简便、经济，尤其适合应用在哺乳动物细胞基因表达与调控的研究中．
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| 关 键 词： | 总RNA 基因组DNA 基因表达与调控 |
| 收稿时间： | 3/1/1993 12:00:00 AM |
| 修稿时间： | 1993/6/13 0:00:00 |
Simultaneous and rapid purification of total cytoplasmic RNA and genomic DNA from small numbers of transfected mammalian cells |
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| Zhang Hongquan,Wang Huixin,Zhou Tingchong and Wang Yunling.Simultaneous and rapid purification of total cytoplasmic RNA and genomic DNA from small numbers of transfected mammalian cells[J].Progress In Biochemistry and Biophysics,1994,21(2):165-166. |
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| Authors: | Zhang Hongquan Wang Huixin Zhou Tingchong and Wang Yunling |
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| Institution: | Inst. Bas. Med. Sci, Acad. Mil. Med. Sci;Inst. Bas. Med. Sci, Acad. Mil. Med. Sci;Inst. Bas. Med. Sci, Acad. Mil. Med. Sci;Inst. Bas. Med. Sci, Acad. Mil. Med. Sci |
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| Abstract: | A protocol by using 4 mol / L LiCl phasing the DNA and RNA could lead to simultaneous and rapid purification of total cytoplasmic RNA and genomic DNA from small numbers of transfected mammaliam cells. Comparing with other methods, this protocol shows rapid. easy and economic, and can be used in many aspects especially in the studies of mammalian cell gene expression and regulation. |
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| Keywords: | total cytoplasmic RNA genomic DNA gene expression and regulation |
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