| 帕金森病裸DNA法基因治疗的实验研究 |
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| 引用本文： | 曹蕾,郑仲承,刘新垣,刘振国,赵迎春,陈生弟,蒋芝华,周长福.帕金森病裸DNA法基因治疗的实验研究[J].生物化学与生物物理进展,1994,21(4):369-369. |
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| 作者姓名： | 曹蕾 郑仲承 刘新垣 刘振国 赵迎春 陈生弟 蒋芝华 周长福 |
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| 作者单位： | 中国科学院上海生物化学研究所;中国科学院上海生物化学研究所;中国科学院上海生物化学研究所;上海瑞金医院;上海瑞金医院;上海瑞金医院;中国科学院上海生理研究所;中国科学院上海生理研究所 |
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| 摘 要： | 采用体内裸DNA基因治疗帕金森病(PD)取得显著疗效.将酪氨酸羟比酶(TH)基冈表达质粒与Lipofectin形成的复合物立体定位注射于PD模型鼠纹状体,显著改善了PD鼠的不对付旋转行为.免疫组化证实神经细胞表达了外源TH.
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| 关 键 词： | 帕金森病 基因治疗 酪氨酸羟化酶 |
| 收稿时间： | 2/8/1994 12:00:00 AM |
| 修稿时间： | 1994/4/24 0:00:00 |
Experimental Research on Naked DNA Gene Therapy of Parkinson's Disease |
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| Cao Lei,Zheng Zhongcheng,Liu Xinyuan,Liu Zhenguo,Zhao Yingchun,Chen Shengdi,Jiang Zhihua and Zhou Changfu.Experimental Research on Naked DNA Gene Therapy of Parkinson's Disease[J].Progress In Biochemistry and Biophysics,1994,21(4):369-369. |
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| Authors: | Cao Lei Zheng Zhongcheng Liu Xinyuan Liu Zhenguo Zhao Yingchun Chen Shengdi Jiang Zhihua and Zhou Changfu |
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| Institution: | Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Biochemistry, Academia Sinica |
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| Abstract: | In vivo naked DNA gene transfer method was used in gene therapy of Parkinson's disease (PD). The complex of rat tyrosine hydroxy-lase (TH) expression plasmid and Lipofectin was injected stereotaxically into striatum of PD rat model. The asymmetric rotational behavior was reduced substantially and quickly. On the third day after injection, drug-induced rotation decreased 50% compared with pretreatment scores. Immunohistochemical staining showed TH-positive nerve cells in striatum of injection side, which indicated that TH gene was uptaked and expressed by nerve cells. These preliminary results have general implications for the application of naked DNA transfer techpique in gene therapy of human neurological disease and specific implications for PD. |
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| Keywords: | Parkinson's disease gene therapy tyrosine hydroxylase |
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