| circPVT1通过miR-24-3p/let-7a-5p/FSCN1轴促进鼻咽癌细胞侵袭迁移 |
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| 引用本文： | 莫勇真,王裕民,范春梅,晏其佳,曾朝阳,蒋卫红,熊芳.circPVT1通过miR-24-3p/let-7a-5p/FSCN1轴促进鼻咽癌细胞侵袭迁移[J].生物化学与生物物理进展,2023,50(8):1946-1958. |
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| 作者姓名： | 莫勇真 王裕民 范春梅 晏其佳 曾朝阳 蒋卫红 熊芳 |
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| 作者单位： | 1)国家老年疾病临床医学研究中心,中南大学湘雅医院耳鼻咽喉头颈外科;2)国家卫健委癌变原理重点实验室和教育部癌变与侵袭原理重点实验室,中南大学肿瘤研究所,1)国家老年疾病临床医学研究中心,中南大学湘雅医院耳鼻咽喉头颈外科,2)国家卫健委癌变原理重点实验室和教育部癌变与侵袭原理重点实验室,中南大学肿瘤研究所,1)国家老年疾病临床医学研究中心,中南大学湘雅医院耳鼻咽喉头颈外科,2)国家卫健委癌变原理重点实验室和教育部癌变与侵袭原理重点实验室,中南大学肿瘤研究所,1)国家老年疾病临床医学研究中心,中南大学湘雅医院耳鼻咽喉头颈外科,1)国家老年疾病临床医学研究中心,中南大学湘雅医院耳鼻咽喉头颈外科 |
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| 基金项目： | 国家自然科学基金（82203496，82002239）和湖南省自然科学基金（2023JJ30955，2021JJ41027）资助项目。 |
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| 摘 要： | 目的 鼻咽癌是一种来源于鼻咽上皮的恶性肿瘤，其临床特征之一是易发生淋巴转移，但是目前鼻咽癌转移的分子机制尚未阐明。circPVT1是由PVT1基因2号外显子反向拼接形成的环状RNA (circRNA)，在多种肿瘤中表达上调，本文探讨了circPVT1在鼻咽癌侵袭迁移中的作用和分子机制。方法 通过RT-qPCR检测circPVT1及其下游miRNA和FSCN1在鼻咽癌细胞的表达情况，Transwell和划痕愈合实验检测circPVT1对鼻咽癌细胞侵袭迁移的影响，RNA pull-down实验检测circPVT1结合的miRNA，双荧光素酶报告实验检测miR-24-3p和let-7a-5p靶向抑制FSCN1 mRNA表达。结果 在鼻咽癌细胞中过表达circPVT1可以促进鼻咽癌细胞侵袭迁移，而敲低circPVT1则可以抑制鼻咽癌细胞的侵袭迁移。进一步研究发现，circPVT1可以通过竞争性吸附miR-24-3p和let-7a-5p，上调FSCN1的表达，从而促进鼻咽癌细胞的侵袭迁移。结论 circPVT1通过miR-24-3p/let-7a-5p/FSCN1轴促进鼻咽癌细胞侵袭迁移，证实c...
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| 关 键 词： | 鼻咽癌 circPVT1 侵袭迁移 miR-24-3p let-7a-5p FSCN1 |
| 收稿时间： | 2023/1/9 0:00:00 |
| 修稿时间： | 2023/7/30 0:00:00 |
circPVT1 Promotes Invasion and Migration of Nasopharyngeal Carcinoma Cells Through The miR-24-3p/let-7a-5p/FSCN1 Axis |
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| MO Yong-Zhen,WANG Yu-Min,FAN Chun-Mei,YAN Qi-Ji,ZENG Zhao-Yang,JIANG Wei-Hong and XIONG Fang.circPVT1 Promotes Invasion and Migration of Nasopharyngeal Carcinoma Cells Through The miR-24-3p/let-7a-5p/FSCN1 Axis[J].Progress In Biochemistry and Biophysics,2023,50(8):1946-1958. |
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| Authors: | MO Yong-Zhen WANG Yu-Min FAN Chun-Mei YAN Qi-Ji ZENG Zhao-Yang JIANG Wei-Hong and XIONG Fang |
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| Institution: | 1)National Clinical Research Center for Geriatric Diseases, Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha 410078, China;2)NHC Key Laboratory of Carcinogenesis and Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China,1)National Clinical Research Center for Geriatric Diseases, Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha 410078, China,2)NHC Key Laboratory of Carcinogenesis and Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China,1)National Clinical Research Center for Geriatric Diseases, Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha 410078, China,2)NHC Key Laboratory of Carcinogenesis and Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Cancer Research Institute, Central South University, Changsha 410078, China,1)National Clinical Research Center for Geriatric Diseases, Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha 410078, China,1)National Clinical Research Center for Geriatric Diseases, Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, Changsha 410078, China |
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| Abstract: | Objective The study aims to explore the role and molecular mechanism of circPVT1 in invasion and migration of nasopharyngeal carcinoma (NPC), a malignant tumor originating from nasopharyngeal epithelium with the clinical feature of lymph node metastasis. circPVT1, a circular RNA formed by back splicing of exon 2 of PVT1 gene, has been found to be upregulated in various tumors and plays an important role in tumorigenesis. The study investigated the role and molecular mechanism of circPVT1 in invasion and migration of NPC cells.Methods We detected the expression of circPVT1 and its downstream miRNAs and FSCN1 in NPC cells using RT-qPCR, and evaluated the effect of circPVT1 on NPC cell invasion and migration using Transwell and scratch healing experiments. RNA pull-down experiment was used to detect the miRNAs bound by circPVT1, and the dual luciferase reporter assay was used to detect the targeting of FSCN1 mRNA by miR-24-3p and let-7a-5p.Results The study found that overexpression of circPVT1 in NPC cells can promote invasion and migration, while knockdown of circPVT1 can inhibit NPC cells invasion and migration. Further studies revealed that circPVT1 upregulates the expression of FSCN1 by competitively adsorbing miR-24-3p and let-7a-5p.Conclusion circPVT1 promotes invasion and migration of NPC cells through the miR-24-3p/let-7a-5p/FSCN1 axis, and is an important driving factor in the development of NPC. |
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| Keywords: | nasopharyngeal carcinoma circPVT1 invasion and migration miR-24-3p let-7a-5p FSCN1 |
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