毛细管电泳-激光诱导荧光分析血清多胺的研究

为进一步探讨多胺的生物学作用，建立了毛细管电泳-激光诱导荧光(λ_ex=488 nm,λ_em=513 nm)分析血清多胺方法.在碱性介质中，多胺与荧光素异硫氰酸酯进行衍生化反应，硼酸盐(pH 8.6)作为运行缓冲液，运行电压20 kV，腐胺、精胺、精脒和1，6-己二胺（内标）在8 min内达到基线分离.考察了方法的线性范围、稳定性、检测限和方法的回收率等，方法具有样品处理简单，灵敏度高，速度快等特点.用于正常对照大鼠和肿瘤大鼠血清多胺的测定.结果提示：实验组肿瘤大鼠血清精胺和精脒水平显著高于正常对照大鼠和实验组未出现肿瘤大鼠血清精胺和精脒水平（P＜0.05），正常对照组大鼠和实验组未出现肿瘤大鼠血清精胺和精脒水平间无显著性差异（P＞0.05），各组间血清腐胺水平均无显著性差异（P＞0.05）.

The Determination of Polyamines in Serum by Capillary Electrophoresis with Laser-induced Fluorescent Detector

To explore the biological functions of polyamines, the analytical method of polyamines by capillary electrophoresis with laser-induced fluorescence detector (LIF) was developed. After derivatization of polyamines with isothiocyanatefluorescin, using borate (pH 8.6) as running buffer, putrescine, spermine, spermidine and 1,6-diaminohexane were separated successfully within 8 min at the voltage of 20 kV. The linear range, stability, detection limit and recovery were examined, the method was simple, sensitive and rapid. The results of polyamines in serum showed: The levels of spermine and spermidine were significantly higher in rat serum of tumor than in rat serum of control group and non-tumor group. There was no markedly difference between the levels of spermine and spermidine in rat serum of control group and in rat serum of non-tumor group. The levels of putrescine in serum were no obviously difference between any groups.