| 游离DNA研究进展及标准化的必要性 |
| |
| 引用本文： | 方艳,刘争,高颖,邢德纯,董莲华,杨靖亚.游离DNA研究进展及标准化的必要性[J].生物化学与生物物理进展,2021,48(9):1042-1051. |
| |
| 作者姓名： | 方艳 刘争 高颖 邢德纯 董莲华 杨靖亚 |
| |
| 作者单位： | 2）中国计量科学研究院前沿计量科学中心，北京 100029,2）中国计量科学研究院前沿计量科学中心，北京 100029,2）中国计量科学研究院前沿计量科学中心，北京 100029,2）中国计量科学研究院前沿计量科学中心，北京 100029,2）中国计量科学研究院前沿计量科学中心，北京 100029,1）上海海洋大学食品学院，上海 201306 |
| |
| 摘 要： | 游离DNA (cell free DNA,cfDNA)作为一种新型分子标志物在疾病预测、肿瘤防治等方面的作用日益突出.但在实现其广泛的临床应用前仍存在诸多障碍,主要是cfDNA相关的标准较少,导致目前的研究报道缺乏可比性,检测结果缺乏稳定的可重复性,从而降低了临床应用的可靠性.本综述集中阐述了近年来cfDNA的相关研究进展,包括cfDNA的样本来源、样本保存、样本提取、定性定量检测和应用,并分析比较了cfDNA研究的各个环节采用的方法及其优缺点,对其中存在的问题进行了剖析和总结,指明了cfDNA相关标准制定的迫切性.
|
| 关 键 词： | cfDNA 数字PCR 提取 检测 |
| 收稿时间： | 2020/10/22 0:00:00 |
| 修稿时间： | 2021/2/8 0:00:00 |
Research Progress of Free DNA and The Necessity of Standardization |
| |
| FANG Yan,LIU Zheng,GAO Ying,XING De-Chun,DONG Lian-Hua and YANG Jing-Ya.Research Progress of Free DNA and The Necessity of Standardization[J].Progress In Biochemistry and Biophysics,2021,48(9):1042-1051. |
| |
| Authors: | FANG Yan LIU Zheng GAO Ying XING De-Chun DONG Lian-Hua and YANG Jing-Ya |
| |
| Institution: | 2)Center for Frontier Metrology, National Institute of Metrology, Beijing 100029, China,2)Center for Frontier Metrology, National Institute of Metrology, Beijing 100029, China,2)Center for Frontier Metrology, National Institute of Metrology, Beijing 100029, China,2)Center for Frontier Metrology, National Institute of Metrology, Beijing 100029, China,2)Center for Frontier Metrology, National Institute of Metrology, Beijing 100029, China,1)College of Food Sciences & Technology, Shanghai Ocean University, Shanghai 201306, China |
| |
| Abstract: | As a new molecular marker, free DNA (cell free DNA, cfDNA) plays an increasingly important role in disease prediction, tumor prevention and treatment. However, there are still many obstacles before achieving a wide range of clinical application, mainly due to the lack of cfDNA-related standards, resulting in the lack of comparability of current research reports and the lack of stable repeatability of test results, thus reducing the reliability of clinical application. This review focuses on the related research progress of cfDNA in recent years, including sample source, sample preservation, sample extraction, qualitative and quantitative detection and application of cfDNA. We analyze and compare the various methods used in each link of cfDNA research and their advantages and disadvantages, analyze and summarize the problems existing in each link, and put forward the urgency of the formulation of cfDNA related standards. |
| |
| Keywords: | cfDNA digital PCR extract detection |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 |
| 点击此处可从《生物化学与生物物理进展》下载免费的PDF全文 |
|
