鲨鱼软骨制剂抑制血管生成的研究

以鲨鱼软骨为原料，经盐酸胍抽提，丙酮分级沉淀, 超滤等步骤得到鲨鱼软骨制剂(shark cartilage preparation,SCP). 利用整装细胞扫描电镜方法测定SCP对血管内皮细胞骨架系统的影响，体外细胞迁移实验测定它对内皮细胞迁移的抑制效应，及鸡胚绒毛尿囊膜实验测定对血管生成的抑制效应. 结果表明SCP能显著抑制内皮细胞的骨架形成；显著抑制内皮细胞的迁移，并有明显的浓度依赖关系；显著抑制鸡胚绒毛尿囊膜的血管生成. 细胞骨架是细胞分裂增殖及运动迁移的基础，血管内皮细胞的运动迁移又是血管生成的基础，因此SCP的作用机理可能是通过抑制细胞骨架的形成，抑制内皮细胞的运动迁移，从而抑制血管生成.

The Inhibitory Effect of Shark Cartilage Preparation on Angiogenesis

Shark cartilage preparation (SCP) was extracted from shark cartilage by the method of guanidine hydrochloride extraction, acetone fractional precipitation, and ultrafiltration. The effect of SCP on the cytoskeleton of endothelial cell was investigated by the whole cell scanning electron microscopy. The effects of SCP on endothelial cell migration and angiogenesis were measured by the cell migration experiment and the chicken embryo chorioallantoic membrane experiment, respectively. The results showed that the cytoskeleton of endothelial cell was wrinkled, and SCP inhibited the migration of endothelial cell, the inhibitory rate was concentration dependent. SCP also inhibited the angiogenesis in the chorioallantoic membrane of chicken embryos significantly. These results suggest that SCP has the inhibitory effect on angiogenesis, and the mechanism may be that SCP inhibits the cytoskeleton of endothelial cell, so inhibits the cell's migration, and so inhibits angiogenesis.