Cre/LoxP系统在转基因小鼠上的应用策略

Cre/LoxP位点特异重组酶系统已发展为在体内外进行遗传操作的一个新的有力工具.该系统在转基因小鼠上的应用，可使转基因的表达或靶基因的缺失/突变的位点特异DNA重组不仅发生在小鼠发育的某一阶段或特定的组织器官,而且,若与控制Cre表达或功能的诱导系统结合，则可以时空方式体现.这些基于重组的策略可能对基因功能的研究和人类疾病的动物模型的建立产生深刻影响.

Strategies and Applications of the Cre/LoxP System in Transgenic Mice

Cre site-specific DNA recombinase system has been developed as a novel powerful tools for manipulating DNA both in vitro and in vivo. It has been used in transgenic mice to induce site-specific DNA recombination leading to gene expression or deletion/mutation not only in a tissue-specific or at a certain stage of development, by combining with inducible systems for controlling Cre expression or function, but also in temporally and spatially manner. These recombination-based strategies are likely to have a profound impact on study of gene function and the generation of animal models of human diseases.