| PEDV S蛋白B细胞抗原表位的筛选和鉴定 |
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| 引用本文： | 孙东波,朗洪武,时洪艳,陈建飞,崔晓辰,王承宝,佟有恩,冯 力.PEDV S蛋白B细胞抗原表位的筛选和鉴定[J].生物化学与生物物理进展,2007,34(9):971-977. |
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| 作者姓名： | 孙东波 朗洪武 时洪艳 陈建飞 崔晓辰 王承宝 佟有恩 冯 力 |
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| 作者单位： | 1. 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,猪传染病研究室,哈尔滨,150001
2. 中国兽医药品监察所,北京,100081 |
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| 摘 要： | 以猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)S基因的免疫优势区S1(1～2367bp)为靶基因,利用基于gⅢ表达外源多肽的fd丝状噬菌体展示系统,构建S1基因噬菌体展示肽库,以纯化病毒制备的兔抗PEDV多克隆血清为靶蛋白,对S1基因噬菌体展示肽库进行3轮生物淘选,结果获得3个高亲和力的序列,分别命名为S1P1(248～280位氨基酸)、S1P2(442～499位氨基酸)和S1P3(697～742位氨基酸).ELISA和蛋白质印迹结果显示,S1P1、S1P2和S1P3短肽都能被兔抗PEDV多克隆血清识别,其中S1P3反应性最强.为了进一步揭示S1P1、S1P2和S1P3短肽的抗原性,制备了3个短肽GST融合蛋白和它们串连后GST融合蛋白的单因子血清,间接免疫荧光试验(IFA)结果证实,抗S1P2-GST、S1P3-GST和S1P123-GST融合蛋白的单因子血清能够识别Vero细胞培养物中天然的PEDV.
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| 关 键 词： | 猪流行性腹泻病毒 S蛋白 抗原表位 噬菌体展示 |
| 收稿时间： | 2007/1/26 0:00:00 |
| 修稿时间： | 2007-01-26 |
Screening and Identification of B Cell Antigenic Epitopes of Porcine Epidemic Diarrhea Virus Spike Glycoprotein by Phage Display |
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| Sun Dong-Bo,LANG Hong-Wu,SHI Hong-Yan,CHEN Jian-Fei,CUI Xiao-Chen,WANG Cheng-Bao,TONG You-En and Feng Li.Screening and Identification of B Cell Antigenic Epitopes of Porcine Epidemic Diarrhea Virus Spike Glycoprotein by Phage Display[J].Progress In Biochemistry and Biophysics,2007,34(9):971-977. |
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| Authors: | Sun Dong-Bo LANG Hong-Wu SHI Hong-Yan CHEN Jian-Fei CUI Xiao-Chen WANG Cheng-Bao TONG You-En and Feng Li |
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| Institution: | Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;China Institute of Veterinary Drug Control, Beijing 100081, China;Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China |
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| Abstract: | |
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| Keywords: | porcine epidemic diarrhea virus(PEDV) spike protein antigenic epitope phage display |
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